Recombinant Human PPOX protein

中文名： 原卟啉原氧化酶(PPOX)重组蛋白
别名： PPOX；Protoporphyrinogen oxidase

货号: PA1000-8802

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PPOX
Uniprot No	P50336
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-477aa
氨基酸序列	MGRTVVVLGGGISGLAASYHLSRAPCPPKVVLVESSERLGGWIRSVRGPNGAIFELGPRGIRPAGALGARTLLLVSELGLDSEVLPVRGDHPAAQNRFLYVGGALHALPTGLRGLLRPSPPFSKPLFWAGLRELTKPRGKEPDETVHSFAQRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLGLLLGAGRTPQPDSALIRQALAERWSQWSLRGGLEMLPQALETHLTSRGVSVLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAAPLARALSAITAVSVAVVNLQYQGAHLPVQGFGHLVPSSEDPGVLGIVYDSVAFPEQDGSPPGLRVTVMLGGSWLQTLEASGCVLSQELFQQRAQEAAATQLGLKEMPSHCLVHLHKNCIPQYTLGHWQKLESARQFLTAHRLPLTLAGASYEGVAVNDCIESGRQAAVSVLGTEPNS
预测分子量	66.8 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PPOX重组蛋白的3篇参考文献及其摘要概括：

1. **文献名称**: "Expression and characterization of recombinant human protoporphyrinogen oxidase"

**作者**: Morgan RR, Erger KE, Magee JC

**摘要**: 该研究报道了人源PPOX基因在大肠杆菌中的重组表达与纯化，并分析了重组蛋白的酶动力学特性，发现其催化活性与天然酶相似，为遗传性卟啉症研究提供了工具。

2. **文献名称**: "Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its mechanistic implications"

**作者**: Koch M, Breithaupt C, Kiefersauer R, et al.

**摘要**: 通过解析粘细菌来源的PPOX晶体结构，揭示了其底物结合位点及催化机制，为设计针对PPOX缺陷相关疾病(如急性肝卟啉症)的抑制剂提供了结构基础。

3. **文献名称**: "Functional analysis of PPOX mutations in variegate porphyria using a mammalian expression system"

**作者**: Frank J, McGrath JA, Poh-Fitzpatrick MB, et al.

**摘要**: 研究利用哺乳动物细胞表达系统构建了多种PPOX突变体重组蛋白，发现特定突变导致酶活性显著下降，解释了变异型卟啉症患者的临床表现差异。

注：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对最新研究。如需补充具体发表年份或期刊信息，可进一步说明。

背景信息

Protoporphyrinogen oxidase (PPOX) is a mitochondrial enzyme critical in the heme biosynthesis pathway, catalyzing the oxidation of protoporphyrinogen IX to protoporphyrin IX. This step is essential for subsequent iron insertion to form heme, a cofactor for hemoglobin, cytochromes, and other hemoproteins. Genetic mutations in the *PPOX* gene are linked to variegate porphyria (VP), an autosomal dominant disorder characterized by cutaneous sensitivity and neurological symptoms due to toxic accumulation of porphyrin precursors.

Recombinant PPOX proteins are engineered to study the enzyme’s structure-function relationships, disease mechanisms, and therapeutic interventions. They are typically produced using heterologous expression systems like *E. coli* or mammalian cell lines, enabling high-yield purification for biochemical assays. Recombinant PPOX retains catalytic activity, allowing researchers to investigate enzyme kinetics, substrate specificity, and inhibition by molecules such as acifluorfen, a herbicide known to target PPOX in plants.

In biomedical research, recombinant PPOX serves as a tool for drug screening, particularly for VP treatment strategies. It aids in developing enzyme replacement therapies or small-molecule modulators to restore heme synthesis balance. Additionally, it supports diagnostic applications, such as detecting pathogenic variants or measuring enzyme activity in patient samples. Recent structural studies using recombinant PPOX, often coupled with X-ray crystallography or cryo-EM, have elucidated mechanistic details, including substrate binding and redox cofactor interactions.

Overall, recombinant PPOX is pivotal in advancing porphyria research, therapeutic development, and understanding mitochondrial enzymology, bridging gaps between genetic defects and clinical manifestations.