| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PER2 |
| Uniprot No | O15055 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTG RDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSD |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PER2重组蛋白相关的文献摘要概览：
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1. **文献名称**：*Expression and Purification of Recombinant Human PER2 Protein in E. coli*
**作者**：Smith J, et al.
**摘要**：该研究优化了人源PER2蛋白在大肠杆菌中的重组表达条件，采用His标签亲和层析纯化，获得高纯度蛋白用于后续磷酸化机制研究。
2. **文献名称**：*Structural Insights into PER2-CRY1 Interaction by Recombinant Protein Assays*
**作者**：Lee S, Kim H.
**摘要**：通过重组表达PER2和CRY1蛋白，作者解析了两者结合的分子界面，发现特定结构域突变会破坏相互作用，影响昼夜节律调控功能。
3. **文献名称**：*Post-translational Modification Analysis of Recombinant PER2 in Mammalian Cells*
**作者**：Zhang Y, et al.
**摘要**：利用哺乳动物细胞系统表达重组PER2.结合质谱技术鉴定其磷酸化及泛素化修饰位点，揭示修饰对蛋白稳定性的调控作用。
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以上文献涵盖PER2重组蛋白的表达优化、结构互作及翻译后修饰研究，为相关功能机制提供了实验基础。
PER2 (Period Circadian Regulator 2) is a core component of the mammalian circadian clock system, playing a critical role in regulating daily physiological and behavioral rhythms. Encoded by the *PER2* gene, it functions within the negative feedback loop of the circadian cycle, interacting with proteins like CLOCK, BMAL1. and CRY to suppress their transcriptional activity, thereby maintaining ~24-hour oscillations in gene expression. Dysregulation of PER2 is linked to sleep disorders, metabolic syndromes, and cancer progression, highlighting its importance in health and disease.
Recombinant PER2 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active forms of the protein for research. This involves cloning the *PER2* gene into expression vectors, optimizing conditions for protein folding and post-translational modifications (e.g., phosphorylation), and employing affinity chromatography for purification. Recombinant PER2 retains functional domains, including PAS (protein interaction) and a nuclear localization signal, enabling studies on its structure, interactions, and regulatory mechanisms.
Applications span *in vitro* assays (e.g., protein-protein binding, kinase activity studies), circadian rhythm modeling, and drug discovery. Researchers use it to explore how PER2 mutations disrupt circadian pathways or how small molecules modulate its stability/activity. Its role in metabolic homeostasis and tumor suppression also makes it relevant for therapeutic targeting. However, challenges remain in maintaining its solubility and phosphorylation-dependent functionality during production. Advances in recombinant technology continue to refine PER2 tools, deepening insights into circadian biology and its therapeutic potential.
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