关键信息
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基因名
KEAP1
- 应用
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别名
KEAP1；INRF2；KIAA0132；KLHL19；Kelch-like ECH-associated Protein 1
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种属
Human
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表达系统
E. coli
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标签
N-terminal His-Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14145
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表达区间
327~624aa
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氨基酸序列
LIYTAGGYFRQSLSYLEAYNPSDGTWLRLADLQVPRSGLAGCVVGGLLYAVGGR NNSPDGNTDSSALDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGC IHHNSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAE CYYPERNEWRMITAMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETE TWTFVAPMKHRRSALGITVHQGRIYVLGGYDGHTFLDSVECYDPDTDTWSEVTR MTSGRSGVGVAVTMEPCRKQIDQQNCTC
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分子量
36.5kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, 5% Trehalose
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KEAP1 (Kelch-like ECH-associated protein 1) is a critical regulator of the cellular antioxidant response, primarily known for its role in modulating the activity of Nrf2 (nuclear factor erythroid 2-related factor 2), a transcription factor that activates the expression of antioxidant genes. KEAP1 functions as a sensor for oxidative stress and maintains cellular redox homeostasis by facilitating the ubiquitination and subsequent degradation of Nrf2 under normal conditions. Dysregulation of the KEAP1-Nrf2 pathway is implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making it a significant focus of research. Recent studies have highlighted the potential therapeutic applications of KEAP1 as a target for drug development, aimed at enhancing Nrf2 activity to combat oxidative stress-related damage. Recombinant KEAP1 protein has been utilized in in vitro studies to better understand its structure-function relationships and interactions with Nrf2 and other cellular partners. By generating and characterizing this recombinant protein, researchers aim to elucidate the molecular mechanisms underlying the KEAP1-Nrf2 signaling pathway and explore novel strategies for the treatment of diseases associated with oxidative stress. Furthermore, understanding KEAP1's regulation and its impact on cellular processes may pave the way for innovative approaches in antioxidant therapy and the development of compounds that can modulate this pathway for therapeutic benefit.
