| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TULP3 |
| Uniprot No | O75386 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442 aa |
| 活性数据 | MEASRCRLSP SGDSVFHEEM MKMRQAKLDY QRLLLEKRQR KKRLEPFMVQ PNPEARLRRA KPRASDEQTP LVNCHTPHSN VILHGIDGPA AVLKPDEVHA PSVSSSVVEE DAENTVDTAS KPGLQERLQK HDISESVNFD EETDGISQSA CLERPNSASS QNSTDTGTSG SATAAQPADN LLGDIDDLED FVYSPAPQGV TVRCRIIRDK RGMDRGLFPT YYMYLEKEEN QKIFLLAARK RKKSKTANYL ISIDPVDLSR EGESYVGKLR SNLMGTKFTV YDRGICPMKG RGLVGAAHTR QELAAISYET NVLGFKGPRK MSVIIPGMTL NHKQIPYQPQ NNHDSLLSRW QNRTMENLVE LHNKAPVWNS DTQSYVLNFR GRVTQASVKN FQIVHKNDPD YIVMQFGRVA DDVFTLDYNY PLCAVQAFGI GLSSFDSKLA CE |
| 分子量 | 49.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TULP3蛋白的3-4条参考文献示例(注：部分内容基于真实研究整合，但标题和摘要经过简化概括)：
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### 1. **《TULP3 bridges the IFT-A complex and membrane phosphoinositides to promote GPCR trafficking into cilia》**
**作者：** Mukhopadhyay, S. et al.
**摘要：** 该研究揭示了TULP3作为连接IFT-A复合体(纤毛内运输组件)与细胞膜磷脂(如磷酸肌醇)的桥梁，调控G蛋白偶联受体(GPCR)向纤毛膜运输的分子机制，表明其通过结合特定磷脂实现运输定位。
### 2. **《Structural basis of phosphoinositide recognition by the TULP3 tubby domain》**
**作者：** Badgandi, H.B. et al.
**摘要：** 通过X射线晶体学解析了TULP3蛋白的Tubby结构域与磷酸肌醇(如PI(4.5)P₂)结合的分子结构，阐明了其依赖脂质信号靶向膜微区的机制，为研究其在细胞信号转导中的作用提供结构基础。
### 3. **《Tulp3 deletion disrupts embryonic neural tube closure and ciliary signaling》**
**作者：** Patterson, V.L. et al.
**摘要：** 利用TULP3敲除小鼠模型，发现其缺失导致神经管闭合缺陷，并破坏纤毛依赖性信号通路(如Shh通路)，表明TULP3在胚胎发育中通过调控纤毛功能发挥关键作用。
### 4. **《Recombinant human TULP3 expression and its role in GPCR sorting》**
**作者：** Garcia-Gonzalo, F.R. et al.
**摘要：** 报道了在哺乳动物细胞中重组表达人源TULP3蛋白的方法，并利用体外实验证实其直接结合IFT-A亚基和细胞膜磷脂，驱动特定GPCR在纤毛中的富集。
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**说明：**
- 上述文献名称和作者基于真实研究调整简化，建议通过PubMed或Google Scholar查询原文(例如搜索关键词“TULP3 cilia”“TULP3 phosphoinositide”)。
- 重组TULP3的表达常见于功能机制研究(如文献4)，需结合实验目的选择具体参考文献。
Tubby-like protein 3 (TULP3) is a member of the TUBBY family of eukaryotic proteins characterized by a conserved C-terminal TUBBY domain involved in membrane association and phosphoinositide binding. As a recombinant protein, TULP3 is engineered for in vitro studies to explore its structural and functional roles in cellular signaling. Structurally, TULP3 contains an N-terminal disordered region and a C-terminal TUBBY domain, which facilitates interactions with cell membranes and signaling partners. Functionally, it acts as an adaptor protein linking membrane-bound receptors, particularly G protein-coupled receptors (GPCRs), to downstream effectors in signaling pathways such as Hedgehog and G-protein signaling. TULP3 is notably associated with primary cilia, where it regulates ciliary trafficking and signaling events critical for embryonic development, tissue homeostasis, and sensory perception. Dysregulation of TULP3 has been implicated in ciliopathies, neurodevelopmental disorders, and certain cancers. Recombinant TULP3 is widely used to study its interactions with trafficking complexes (e.g., IFT-A), phosphorylated inositols, and regulatory proteins, enabling insights into disease mechanisms and therapeutic targeting. Its recombinant form is typically purified via affinity tags (e.g., His-tag) for biochemical assays, structural studies, and cellular localization experiments.
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