| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CELSR2 |
| Uniprot No | Q9HCU4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于CELSR2重组蛋白的模拟参考文献(内容基于公开研究领域知识整理，具体文献需通过学术数据库验证)：
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1. **文献名称**: *Structural Insights into CELSR2 Cadherin Repeats by Recombinant Protein Expression*
**作者**: Smith A, et al.
**摘要**: 通过重组表达技术纯化CELSR2的胞外结构域，利用X射线晶体学解析其三维结构，揭示其钙离子依赖性黏附界面的关键氨基酸残基，为研究其在平面细胞极性中的作用提供结构基础。
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2. **文献名称**: *Functional Analysis of CELSR2 Variants in Neural Tube Development*
**作者**: Chen L, et al.
**摘要**: 构建CELSR2重组蛋白突变体，通过体外细胞黏附实验和小鼠模型，证明特定结构域突变导致信号传导异常，与神经管闭合缺陷相关，提示CELSR2在胚胎发育中的关键作用。
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3. **文献名称**: *CELSR2 Recombinant Protein Interacts with Wnt/PCP Pathway Components*
**作者**: Tanaka K, et al.
**摘要**: 利用重组CELSR2蛋白进行蛋白质互作筛选，发现其与Wnt/平面细胞极性(PCP)通路中的Vangl2和Fzd3直接结合，支持CELSR2作为PCP信号核心调控分子的假说。
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4. **文献名称**: *Association of CELSR2 Polymorphisms with Cardiovascular Disease: A Biochemical Study*
**作者**: Gupta R, et al.
**摘要**: 通过重组蛋白功能实验验证CELSR2基因多态性对其蛋白稳定性和细胞迁移能力的影响，提出特定突变可能通过改变内皮细胞动力学增加动脉粥样硬化风险。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "CELSR2 recombinant protein" 或结合具体研究领域筛选最新文献。
CELSR2 (Cadherin EGF LAG Seven-Pass G-Type Receptor 2) is a member of the non-classical cadherin superfamily and plays a critical role in planar cell polarity (PCP) signaling, a conserved pathway regulating tissue organization and morphogenesis during embryonic development. As a seven-pass transmembrane receptor, CELSR2 features multiple extracellular cadherin repeats, EGF-like domains, and a laminin G motif, enabling cell-cell adhesion and signal transduction. It interacts with core PCP components like Frizzled and Vangl to coordinate polarized cellular behaviors, such as oriented cell division, collective migration, and tissue patterning.
Recombinant CELSR2 proteins are engineered to study its structural and functional properties in vitro. Typically produced in mammalian or insect expression systems, these proteins retain key domains for ligand binding and pathway activation. Researchers utilize them to investigate CELSR2's role in neural tube closure, cardiovascular development, and epithelial tissue alignment, with defects linked to congenital disorders like spina bifida. Additionally, CELSR2 dysregulation is implicated in cancer metastasis and ciliopathies, making recombinant forms valuable for drug screening and mechanistic studies. Recent advances in cryo-EM have leveraged purified CELSR2 to resolve its oligomeric state and interaction interfaces, offering insights into PCP pathway architecture. Its recombinant versions also serve as antigens for antibody development in diagnostic applications.
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