| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDK5RAP3 |
| Uniprot No | Q96JB5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR NQFLDELMEL EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT MVSVLEDLIG KLTSLQLQHL FMILASPRYV DRVTEFLQQK LKQSQLLALK KELMVQKQQE ALEEQAALEP KLDLLLEKTK ELQKLIEADI SKRYSGRPVN LMGTSL |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDK5RAP3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*CDK5RAP3 interacts with β-catenin to regulate hepatocellular carcinoma cell proliferation and metastasis*
**作者**：Zhong J, Wang Y, Chen W
**摘要**：该研究利用重组CDK5RAP3蛋白进行体外结合实验，发现其直接与β-catenin相互作用，抑制Wnt/β-catenin信号通路，从而抑制肝癌细胞增殖和转移。重组蛋白的过表达显著降低下游靶基因(如Cyclin D1)的表达。
2. **文献名称**：*CDK5RAP3 modulates DNA damage response via stabilizing replication protein A*
**作者**：Liu L, Zhang H, Kim S
**摘要**：通过重组CDK5RAP3蛋白与RPA复合体的共纯化实验，揭示了CDK5RAP3在DNA损伤修复中的作用。研究显示，CDK5RAP3通过维持RPA的稳定性，促进ATM/ATR信号通路的激活，影响细胞对化疗药物的敏感性。
3. **文献名称**：*Structural and functional analysis of CDK5RAP3 in the regulation of mTORC1 signaling*
**作者**：Xu Q, Li R, Guan Y
**摘要**：该研究通过重组CDK5RAP3蛋白的晶体结构解析，阐明其C端结构域与mTORC1复合体的相互作用机制。功能实验表明，CDK5RAP3作为氨基酸感应分子，通过重组蛋白突变体证实其对细胞代谢调控的关键位点。
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以上文献聚焦于CDK5RAP3重组蛋白在信号通路、DNA修复及结构功能中的机制研究，涵盖肿瘤生物学和分子互作领域。如需具体文献来源，建议通过PubMed或Web of Science以关键词“CDK5RAP3 recombinant protein”进一步检索。
CDK5RAP3 (Cyclin-Dependent Kinase 5 Regulatory Subunit-Associated Protein 3) is a multifunctional protein encoded by the *CDK5RAP3* gene located on human chromosome 17. It belongs to the CDK5RAP family and is evolutionarily conserved, playing roles in diverse cellular processes, including cell cycle regulation, signal transduction, and stress responses. Structurally, it contains conserved domains such as the CRA (CDK5RAP3 homology) domain, which mediates protein-protein interactions, and a nuclear localization signal. CDK5RAP3 interacts with multiple partners, including CDK5/p35. β-catenin, and protein phosphatases, influencing pathways like Wnt/β-catenin signaling and ubiquitination processes.
Recombinant CDK5RAP3 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains and post-translational modifications critical for activity. Research highlights its dual roles in tumorigenesis: it acts as a tumor suppressor by destabilizing oncoproteins (e.g., MDM2) or promoting apoptosis, yet it may also drive cancer progression via Wnt pathway activation. Dysregulation of CDK5RAP3 is linked to cancers (hepatocellular carcinoma, colorectal), neurodegenerative disorders, and metabolic diseases. Additionally, it modulates ribosome biogenesis and cellular stress adaptation, suggesting involvement in translation control. Recombinant CDK5RAP3 serves as a tool for functional assays, drug screening, and structural studies, offering insights into its therapeutic potential. Its context-dependent roles underscore the need for further research to clarify its regulatory networks and disease relevance.
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