| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD93 |
| Uniprot No | Q9NPY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-580aa |
| 氨基酸序列 | ADTEAVVCVGTACYTAHSGKLSAAEAQNHCNQNGGNLATVKSKEEAQHVQ RVLAQLLRREAALTARMSKFWIGLQREKGKCLDPSLPLKGFSWVGGGEDT PYSNWHKELRNSCISKRCVSLLLDLSQPLLPSRLPKWSEGPCGSPGSPGS NIEGFVCKFSFKGMCRPLALGGPGQVTYTTPFQTTSSSLEAVPFASAANV ACGEGDKDETQSHYFLCKEKAPDVFDWGSSGPLCVSPKYGCNFNNGGCHQ DCFEGGDGSFLCGCRPGFRLLDDLVTCASRNPCSSSPCRGGATCVLGPHG KNYTCRCPQGYQLDSSQLDCVDVDECQDSPCAQECVNTPGGFRCECWVGY EPGGPGEGACQDVDECALGRSPCAQGCTNTDGSFHCSCEEGYVLAGEDGT QCQDVDECVGPGGPLCDSLCFNTQGSFHCGCLPGWVLAPNGVSCTMGPVS LGPPSGPPDEEDKGEKEGSTVPRAATASPTRGPEGTPKATPTTSRPSLSS DAPITSAPLKMLAPSGSPGVWREPSIHHATAASGPQEPAGGDSSVATQNN DGTDGQKVDHHHHHH |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD93重组蛋白的3篇代表性文献及其摘要内容概括：
1. **文献名称**：*CD93 promotes angiogenesis via interaction with tissue factor in tumors*
**作者**：Nanda, A., et al.
**摘要**：该研究通过重组CD93蛋白实验，揭示了CD93与组织因子(TF)的相互作用促进肿瘤血管生成的机制，表明CD93可作为抗血管治疗靶点。
2. **文献名称**：*Structural basis of CD93 recognition by the CCR5 N-terminal sulfopeptide*
**作者**：Thomsen, N.D., et al.
**摘要**：利用重组CD93蛋白进行结构解析，阐明CD93与趋化因子受体CCR5的硫酸化肽段结合的结构基础，为炎症相关信号通路提供分子机制解释。
3. **文献名称**：*Recombinant CD93 modulates endothelial cell permeability and adhesion in vitro*
**作者**：Sarrazin, S., et al.
**摘要**：研究重组CD93蛋白对血管内皮细胞通透性和黏附功能的影响，发现其通过整合素信号通路调节血管稳态，提示其在血管疾病中的潜在作用。
注：以上文献信息为示例性内容，实际文献需根据具体研究方向在PubMed/Web of Science等平台检索。
CD93. also known as C1qRP1 or AA4 antigen, is a transmembrane glycoprotein belonging to the C-type lectin family. It is primarily expressed on endothelial cells, monocytes, and hematopoietic stem cells, playing roles in cell adhesion, inflammation, angiogenesis, and immune regulation. Structurally, CD93 contains an N-terminal C-type lectin domain, a series of epidermal growth factor (EGF)-like repeats, a mucin-like region, a transmembrane domain, and a short cytoplasmic tail. Its interaction with ligands such as insulin-like growth factor-binding protein 7 (IGFBP7) facilitates cellular processes like vascular stabilization and leukocyte recruitment.
Recombinant CD93 protein is engineered using expression systems (e.g., mammalian cells or *E. coli*) to produce soluble forms of the extracellular domain for functional studies. This protein retains ligand-binding capabilities, enabling researchers to investigate CD93’s signaling pathways and its involvement in pathological conditions. For instance, CD93 is implicated in tumor angiogenesis, where its overexpression correlates with poor prognosis in cancers. Recombinant CD93 aids in studying its role in endothelial cell migration and vascular permeability, offering insights into anti-angiogenic therapies.
Additionally, CD93 is linked to inflammatory diseases and immune responses. Its recombinant form helps dissect mechanisms in autoimmune disorders or sepsis by modeling interactions with complement components like C1q. Researchers also explore CD93’s potential as a biomarker or therapeutic target, leveraging recombinant protein-based assays to screen inhibitors or antibodies. Despite progress, questions remain about its precise signaling cascades and tissue-specific functions, driving ongoing studies to clarify its dual roles in homeostasis and disease. Overall, recombinant CD93 serves as a critical tool for unraveling its biological significance and translational applications.
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