首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子

Recombinant Human MCP4 protein

  • 中文名: 单核细胞趋化蛋白4(MCP4)重组蛋白
  • 别    名: MCP4;MCP4;NCC1;SCYA13;C-C motif chemokine 13
货号: PA1000-8151
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MCP4
Uniprot NoQ99616
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间24-98aa
氨基酸序列QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
预测分子量9 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MCP4(膜辅蛋白4.CD46)重组蛋白研究的示例参考文献(注:内容为模拟概括,实际文献需通过学术数据库查询):

---

1. **文献名称**:*"Recombinant Human Membrane Cofactor Protein (MCP-4) Suppresses Complement Activation in vitro"*

**作者**:Atkins, R.C. et al.

**摘要**:研究通过昆虫细胞表达系统成功制备重组人MCP4蛋白,验证其抑制补体C3转化酶的功能,证明其在体外模型中可有效减少补体介导的细胞损伤。

2. **文献名称**:*"Structural Analysis of CD46/MCP-4 and Its Interaction with Pathogens"*

**作者**:Seya, T., et al.

**摘要**:解析重组MCP4的晶体结构,揭示其与麻疹病毒血凝素蛋白的结合机制,为开发抗病毒药物提供结构基础。

3. **文献名称**:*"Expression and Functional Characterization of Soluble MCP-4 in Autoimmune Disease Models"*

**作者**:Sastry, K., et al.

**摘要**:利用哺乳动物细胞表达可溶性MCP4重组蛋白,并在小鼠模型中验证其通过调控补体通路减轻自身免疫性肾炎的疗效。

4. **文献名称**:*"Engineering MCP-4 as a Decoy Receptor for Measles Virus Therapy"*

**作者**:Cattaneo, R., et al.

**摘要**:改造重组MCP4作为麻疹病毒受体诱饵,体外实验显示其可阻断病毒入侵宿主细胞,提示其作为抗病毒策略的潜力。

---

建议通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词“recombinant MCP4/CD46”获取真实文献。

背景信息

MCP4 (Monocyte Chemoattractant Protein-4), also known as CCL13. is a small secretory protein belonging to the CC chemokine family. It plays a regulatory role in immune responses by recruiting monocytes, T cells, eosinophils, and dendritic cells via interaction with chemokine receptors, particularly CCR2 and CCR3. Structurally, MCP4 contains a conserved CC motif and shares homology with other MCP family members (e.g., MCP-1/CCL2). Its expression is induced by pro-inflammatory cytokines like TNF-α or IL-1β in endothelial cells, fibroblasts, and macrophages during infection or tissue injury.

Recombinant MCP4 proteins are engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to study its biological functions or therapeutic potential. These proteins are typically produced with tags (e.g., His-tag) for purification and may lack post-translational modifications unless expressed in eukaryotic systems. Researchers utilize recombinant MCP4 to investigate chemotaxis mechanisms, immune cell trafficking in diseases (e.g., asthma, rheumatoid arthritis), or receptor signaling pathways. Its involvement in chronic inflammation and fibrosis has also made it a target for drug development, with studies exploring neutralizing antibodies or receptor antagonists. Quality-controlled batches ensure consistency in experimental reproducibility for both in vitro assays and preclinical models.

客户数据及评论

折叠内容

大包装询价

×