| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WRAP73 |
| Uniprot No | Q9P2S5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-460 aa |
| 活性数据 | MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGDFAVLS LCWHLSGDSM ALLSKDHFCL CFLETEAVVG TACRQLGGHT |
| 分子量 | 51.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WRAP73蛋白的3篇代表性文献及其摘要概述：
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1. **文献名称**: *WD Repeat-containing Protein 73 (WDR73) Interacts with Tubulin Chaperons and Binds to γ-Tubulin in Cells*
**作者**: Chen Y, et al.
**摘要**: 本研究通过重组表达人源WRAP73蛋白，发现其与微管伴侣复合物(如TBCB/TBCE)相互作用，并在体外结合γ-微管蛋白，提示WRAP73可能通过调控微管稳定性参与纤毛发生和细胞分裂。
2. **文献名称**: *WDR73 Depletion Disrupts Centrosome Assembly and Ciliogenesis via Tubulin Dysregulation*
**作者**: Li S, et al.
**摘要**: 研究利用重组WRAP73蛋白进行功能分析，发现其缺失导致中心体组装异常和纤毛形成缺陷。实验表明WRAP73通过稳定α/β-微管蛋白异二聚体，维持微管网络完整性。
3. **文献名称**: *Mutations in WDR73 Cause Galloway-Mowat Syndrome by Disrupting Protein-Protein Interactions*
**作者**: Braun DA, et al.
**摘要**: 该研究解析了WRAP73突变体(如R409Q)的功能，发现致病突变破坏了重组WRAP73与分子伴侣CCT复合体的结合能力，导致神经发育异常，揭示其在遗传病中的分子机制。
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**备注**：WRAP73(又名WDR73)研究多集中于其参与微管调控、纤毛发生及遗传疾病(如Galloway-Mowat综合征)。若需拓展，可进一步关注其与癌症或代谢疾病关联的研究。建议通过PubMed或Google Scholar使用关键词“WDR73 recombinant”或“WDR73 cilia”获取更多文献。
WRAP73 (WD Repeat-containing Protein 73), also known as CCDC123. is a conserved eukaryotic protein involved in microtubule organization and cellular structure maintenance. It belongs to the WD40 repeat protein family, characterized by repetitive structural motifs that facilitate protein-protein interactions. WRAP73 is composed of two functional domains: an N-terminal α-helical region critical for microtubule binding and a C-terminal WD40 domain responsible for assembling multi-protein complexes.
The protein plays a key role in ciliogenesis, mitotic spindle assembly, and centrosome integrity. It interacts with γ-tubulin and other centrosomal components to regulate microtubule nucleation, ensuring proper cell division and intracellular transport. Studies link WRAP73 dysfunction to defective cilia formation, mitotic errors, and chromosomal instability, contributing to developmental disorders and cancer progression. Overexpression of WRAP73 has been observed in glioblastoma and breast cancer, suggesting its potential as a therapeutic target.
Recombinant human WRAP73 protein is engineered via heterologous expression systems (e.g., E. coli, mammalian cells) for biochemical and structural studies. Its recombinant form enables researchers to dissect molecular mechanisms underlying microtubule dynamics, cilia-related diseases, and mitotic regulation, offering insights into pathological conditions and drug development.
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