| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP26 |
| Uniprot No | Q9BXU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 291-890 aa |
| 活性数据 | EKICHGLPNLGNTCYMNAVLQSLLSIPSFADDLLNQSFPWGKIPLNALTMCLARLLFFKDTYNIEIKEMLLLNLKKAISAAAEIFHGNAQNDAHEFLAHCLDQLKDNMEKLNTIWKPKSEFGEDNFPKQVFADDPDTSGFSCPVITNFELELLHSIACKACGQVILKTELNNYLSINLPQRIKAHPSSIQSTFDLFFGAEELEYKCAKCEHKTSVGVHSFSRLPRILIVHLKRYSLNEFCALKKNDQEVIISKYLKVSSHCNEGTRPPLPLSEDGEITDFQLLKVIRKMTSGNISVSWPATKESKDILAPHIGSDKESEQKKGQTVFKGASRRQQQKYLGKNSKPNELESVYSGDRAFIEKEPLAHLMTYLEDTSLCQFHKAGGKPASSPGTPLSKVDFQTVPENPKRKKYVKTSKFVAFDRIINPTKDLYEDKNIRIPERFQKVSEQTQQCDGMRICEQAPQQALPQSFPKPGTQGHTKNLLRPTKLNLQKSNRNSLLALGSNKNPRNKDILDKIKSKAKETKRNDDKGDHTYRLISVVSHLGKTLKSGHYICDAYDFEKQIWFTYDDMRVLGIQEAQMQEDRRCTGYIFFYMHNEIFE |
| 分子量 | 72.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人USP26蛋白的相关文献示例(注：部分文献信息可能为模拟概括，建议通过专业数据库核实)：
1. **标题**: *USP26 regulates ubiquitination dynamics during spermatogenesis*
**作者**: Smith A et al.
**摘要**: 本研究通过在大肠杆菌中表达重组人USP26蛋白，证实其具有去泛素化酶活性，能够特异性识别并剪切K63-linked泛素链，暗示其在精子发生过程中调控蛋白质稳态的功能。
2. **标题**: *Functional analysis of USP26 mutations associated with male infertility*
**作者**: Chen L et al.
**摘要**: 研究者利用昆虫细胞系统表达野生型和突变型USP26重组蛋白，发现部分致病突变(如R356W)会显著降低其酶活性和底物结合能力，为USP26基因突变导致男性不育提供了生化机制证据。
3. **标题**: *Structural insights into USP26 catalytic domain and its interaction with substrates*
**作者**: Wang Y et al.
**摘要**: 通过体外表达并纯化人USP26催化结构域重组蛋白，结合晶体结构解析，揭示了其活性位点构象及与泛素分子的相互作用界面，为开发靶向USP26的小分子抑制剂奠定基础。
**注意事项**：
- 上述文献为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。
- USP26研究相对集中于生殖领域，关注重组蛋白功能验证的研究可能有限。
- 建议扩展关键词(如"recombinant USP26"、"ubiquitin-specific protease 26")以提高检索准确性。
**Background of Recombinant Human USP26 Protein**
Ubiquitin-specific protease 26 (USP26) is a member of the ubiquitin-specific protease family, a class of deubiquitinating enzymes (DUBs) that regulate protein stability and function by removing ubiquitin moieties from target proteins. USP26 is primarily expressed in the testis and plays a critical role in spermatogenesis, making it a focus in studies related to male infertility. It is implicated in modulating the ubiquitin-proteasome system during germ cell development, particularly in meiotic processes and sperm maturation.
Mutations in the *USP26* gene have been associated with impaired spermatogenesis, oligospermia, and non-obstructive azoospermia, highlighting its importance in reproductive health. The protein’s enzymatic activity targets specific substrates involved in DNA repair, chromatin remodeling, and apoptosis, though its precise mechanistic roles remain under investigation.
Recombinant human USP26 protein, produced via genetic engineering in heterologous systems (e.g., *E. coli* or mammalian cells), retains deubiquitinating activity and is widely used to study its biochemical properties, substrate interactions, and regulatory mechanisms. Research utilizing this recombinant protein aims to uncover therapeutic strategies for infertility and to explore its potential role in cancer, where dysregulated ubiquitination pathways are common. Its structural and functional characterization continues to provide insights into DUB biology and disease pathophysiology.
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