| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP12 |
| Uniprot No | O75317 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370 aa |
| 活性数据 | MEILMTVSKF ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC RPFREKVLAY KSQPRKKESL LTCLADLFHS IATQKKKVGV IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADIL QEERKQEKQN GRLPNGNIDN ENNNSTPDPT WVHEIFQGTL TNETRCLTCE TISSKDEDFL DLSVDVEQNT SITHCLRGFS NTETLCSEYK YYCEECRSKQ EAHKRMKVKK LPMILALHLK RFKYMDQLHR YTKLSYRVVF PLELRLFNTS GDATNPDRMY DLVAVVVHCG SGPNRGHYIA IVKSHDFWLL FDDDIVEKID AQAIEEFYGL TSDISKNSES GYILFYQSRD |
| 分子量 | 42.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人USP12蛋白的参考文献摘要示例(基于真实研究背景的概括，具体作者和期刊信息为假设性示例)：
1. **文献名称**: *USP12 stabilizes MYC protein by deubiquitination and promotes tumorigenesis*
**作者**: Sun T. et al.
**摘要**: 研究发现USP12通过去泛素化作用稳定MYC蛋白，抑制其降解，增强MYC驱动的转录活性，从而促进肿瘤细胞增殖与存活，为靶向USP12-MYC轴在癌症治疗中的潜在应用提供依据。
2. **文献名称**: *USP12 regulates DNA damage repair by stabilizing RAD51 in Fanconi anemia pathway*
**作者**: Toma M. et al.
**摘要**: 本文揭示USP12通过去泛素化修饰维持RAD51蛋白稳定性，调控范可尼贫血通路中的DNA同源重组修复，缺失USP12会导致基因组不稳定和化疗敏感性增加。
3. **文献名称**: *USP12 interacts with PHF1 to modulate Tau phosphorylation and neurodegeneration*
**作者**: Smith J. & Lee K.
**摘要**: 研究证明USP12与表观调控因子PHF1形成复合物，通过去泛素化作用降低Tau蛋白磷酸化水平，减缓神经退行性疾病中Tau病理进展，提示其在阿尔茨海默病中的调控机制。
4. **文献名称**: *USP12 deubiquitinates β-catenin to potentiate Wnt signaling in stem cell maintenance*
**作者**: Kumar R. et al.
**摘要**: 该研究阐明USP12通过去泛素化β-catenin增强Wnt信号通路活性，维持胚胎干细胞的自我更新能力，为干细胞治疗和再生医学提供新靶点。
注：以上内容为基于USP12已知功能(去泛素化酶、癌症、DNA修复、神经疾病)的假设性文献示例，实际文献需通过数据库(PubMed/Web of Science)以“USP12”为关键词检索验证。
Ubiquitin-specific protease 12 (USP12) is a deubiquitinating enzyme belonging to the ubiquitin-specific protease (USP) family, which regulates protein stability and function by removing ubiquitin chains from target substrates. It is evolutionarily conserved and plays critical roles in diverse cellular processes, including DNA damage repair, epigenetic regulation, and immune response. Structurally, USP12 contains a conserved catalytic domain responsible for its enzymatic activity and N-terminal regulatory domains that mediate protein interactions. Notably, USP12 often functions in conjunction with co-factors such as UAF1 and WDR20. forming a ternary complex essential for its full enzymatic activation and substrate specificity.
Research has linked USP12 to the stabilization of key proteins involved in oncogenesis (e.g., MDM2. AR) and neurodegenerative pathways, highlighting its dual role in promoting tumorigenesis and modulating neuronal health. Its dysregulation is implicated in cancers (e.g., prostate, liver) and neurodegenerative disorders like Alzheimer’s disease. Recombinant human USP12 protein is typically produced in mammalian or insect expression systems to ensure proper post-translational modifications. Purified through affinity chromatography, it serves as a vital tool for in vitro studies to dissect USP12’s biochemical properties, substrate interactions, and inhibitory drug screening. Ongoing studies aim to exploit USP12 as a therapeutic target by developing selective inhibitors or modulators.
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