| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAF3IP1 |
| Uniprot No | Q8TDR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-691 aa |
| 活性数据 | MNAAVVRRTQ EALGKVIRRP PLTEKLLSKP PFRYLHDIIT EVIRMTGFMK GLYTDAEMKS DNVKDKDAKI SFLQKAIDVV VMVSGEPLLA KPARIVAGHE PERTNELLQI IGKCCLNKLS SDDAVRRVLA GEKGEVKGRA SLTSRSQELD NKNVREEESR VHKNTEDRGD AEIKERSTSR DRKQKEELKE DRKPREKDKD KEKAKENGGN RHREGERERA KARARPDNER QKDRGNRERD RDSERKKETE RKSEGGKEKE RLRDRDRERD RDKGKDRDRR RVKNGEHSWD LDREKNREHD KPEKKSASSG EMSKKLSDGT FKDSKAETET EISTRASKSL TTKTSKRRSK NSVEGRKEDN ISAKSLDSIV SGINNEPNQE TTTSEIGTKE ANINSTSISD DNSASLRCEN IQPNPTEKQK GDSTSDAEGD AGPAGQDKSE VPETPEIPNE LSSNIRRIPR PGSARPAPPR VKRQDSMEAL QMDRSGSGKT VSNVITESHN SDNEEDDQFV VEAAPQLSEM SEIEMVTAVE LEEEEKHGGL VKKILETKKD YEKLQQSPKP GEKERSLFES AWKKEKDIVS KEIEKLRTSI QTLCKSALPL GKIMDYIQED VDAMQNELQM WHSENRQHAE ALQQEQRITD CAVEPLKAEL AELEQLIKDQ QDKICAVKAN ILKNEEKIQK MVYSINLTSR R |
| 分子量 | 78.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRAF3IP1蛋白的3-4篇参考文献及其简要摘要：
1. **"TRAF3IP1 mediates Angiotensin II-induced inflammation through JAK2/STAT3 signaling in vascular smooth muscle cells"**
*Authors: Li Y, et al. (2019)*
摘要：研究揭示了TRAF3IP1在血管平滑肌细胞中介导Angiotensin II诱导的炎症反应，通过激活JAK2/STAT3通路促进炎症因子释放，可能在高血压及动脉硬化中起关键作用。
2. **"TRAF3IP1 negatively regulates virus-triggered NF-κB signaling by targeting TRAF3 for degradation"**
*Authors: Wang Q, et al. (2014)*
摘要：发现TRAF3IP1通过泛素-蛋白酶体途径降解TRAF3.负调控病毒感染触发的NF-κB信号通路，揭示其在抗病毒天然免疫应答中的调节机制。
3. **"TRAF3IP1 promotes lupus nephritis by enhancing CD40-mediated NF-κB activation in podocytes"**
*Authors: Li QZ, et al. (2020)*
摘要：发现TRAF3IP1在狼疮性肾炎的足细胞中高表达，通过增强CD40信号驱动的NF-κB活化，促进炎症损伤，提出其作为治疗靶点的潜力。
4. **"Structural basis of TRAF3IP1 interaction with the adaptor protein TAX1BP1"**
*Authors: He X, et al. (2016)*
摘要：解析了TRAF3IP1与TAX1BP1的复合物晶体结构，阐明了其通过特定结构域介导蛋白质相互作用，参与炎症和自噬信号调控的分子机制。
(注：以上文献信息为虚拟示例，实际研究中需根据具体论文内容及数据库核对。)
TRAF3IP1 (TRAF3 Interacting Protein 1), also known as IIP-1 or Act1. is a cytoplasmic adapter protein involved in regulating immune and inflammatory signaling pathways. It contains an N-terminal helix-loop-helix domain, a central coiled-coil domain, and a C-terminal TRAF6-binding motif, facilitating interactions with key signaling molecules like TRAF3. TRAF6. and IL-17 receptors. Functionally, TRAF3IP1 serves as a critical mediator in IL-17-dependent signaling, modulating NF-κB and MAPK pathways to influence pro-inflammatory cytokine production (e.g., IL-6. TNF-α) and immune cell activation.
Its role extends to autoimmune and inflammatory diseases, with studies linking TRAF3IP1 dysregulation to conditions like psoriasis, rheumatoid arthritis, and multiple sclerosis. Structural studies highlight its dual role as a scaffold protein and E3 ubiquitin ligase, orchestrating signal transduction through protein ubiquitination. Recombinant human TRAF3IP1 is widely used in vitro to dissect molecular mechanisms of immune responses, identify therapeutic targets, and screen small-molecule inhibitors. Recent research also explores its involvement in cancer progression and metabolic disorders, underscoring its broad pathophysiological relevance. Genetic knockout models demonstrate its necessity in maintaining immune homeostasis, making it a focus for translational studies aiming to develop precision therapies against chronic inflammation.
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