| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNK1 |
| Uniprot No | Q13470 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-666 aa |
| 活性数据 | MLPEAGSLWL LKLLRDIQLA QFYWPILEEL NVTRPEHFDF VKPEDLDGIG MGRPAQRRLS EALKRLRSGP KSKNWVYKIL GGFAPEHKEP TLPSDSPRHL PEPEGGLKCL IPEGAVCRGE LLGSGCFGVV HRGLWTLPSG KSVPVAVKSL RVGPEGPMGT ELGDFLREVS VMMNLEHPHV LRLHGLVLGQ PLQMVMELAP LGSLHARLTA PAPTPPLLVA LLCLFLRQLA GAMAYLGARG LVHRDLATRN LLLASPRTIK VADFGLVRPL GGARGRYVMG GPRPIPYAWC APESLRHGAF SSASDVWMFG VTLWEMFSGG EEPWAGVPPY LILQRLEDRA RLPRPPLCSR ALYSLALRCW APHPADRPSF SHLEGLLQEA GPSEACCVRD VTEPGALRME TGDPITVIEG SSSFHSPDST IWKGQNGRTF KVGSFPASAV TLADAGGLPA TRPVHRGTPA RGDQHPGSID GDRKKANLWD APPARGQRRN MPLERMKGIS RSLESVLSLG PRPTGGGSSP PEIRQARAVP QGPPGLPPRP PLSSSSPQPS QPSRERLPWP KRKPPHNHPM GMPGARKAAA LSGGLLSDPE LQRKIMEVEL SVHGVTHQEC QTALGATGGD VVSAIRNLKV DQLFHLSSRS RADCWRILEH YQWDLSAASR YVLARP |
| 分子量 | 72.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TNK1蛋白的虚构参考文献示例(注意：TNK1相关研究较少，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Structural and Functional Characterization of Recombinant Human TNK1 Kinase*
**作者**：Smith J, et al.
**摘要**：解析了重组人TNK1蛋白的晶体结构，并证明其通过磷酸化下游靶点调控细胞增殖和凋亡。
2. **文献名称**：*TNK1 Regulates NF-κB Signaling in Inflammatory Responses*
**作者**：Li X, et al.
**摘要**：发现重组TNK1蛋白通过激活NF-κB通路参与炎症反应，为自身免疫疾病治疗提供新靶点。
3. **文献名称**：*Recombinant TNK1 as a Potential Therapeutic Target in Glioblastoma*
**作者**：Wang Y, et al.
**摘要**：证明TNK1在胶质母细胞瘤中高表达，体外重组蛋白实验显示抑制TNK1可减少肿瘤侵袭。
4. **文献名称**：*High-Throughput Screening of TNK1 Inhibitors Using Recombinant Protein Assays*
**作者**：Kim S, et al.
**摘要**：基于重组TNK1蛋白建立药物筛选平台，筛选出多个小分子抑制剂候选物。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词：
`"recombinant TNK1" OR "TNK1 kinase" + structure/function/therapy`
注意确认基因别名(如ACK1可能为同一蛋白)。
TNK1 (TRAF2 and NCK-interacting kinase 1) is a non-receptor tyrosine kinase encoded by the TNK1 gene in humans. It belongs to the ACK family of kinases, characterized by a unique structural organization that includes a kinase domain, a SH3 domain, and a conserved C-terminal region. TNK1 is involved in regulating cellular processes such as cytoskeletal dynamics, cell migration, and apoptosis. It interacts with signaling adaptors like TRAF2 and NCK, linking it to pathways influenced by growth factors, cytokines, and stress signals. Dysregulation of TNK1 has been implicated in cancer, where its overexpression or aberrant activation may promote tumor progression and metastasis.
Recombinant human TNK1 protein is produced using biotechnological methods, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications. This engineered protein retains kinase activity and is utilized in biochemical assays to study TNK1's enzymatic mechanisms, substrate interactions, and regulatory roles. Researchers employ it to screen for kinase inhibitors, investigate signaling crosstalk in disease models, and clarify TNK1's physiological and pathological functions. Its study contributes to understanding tyrosine kinase biology and potential therapeutic targeting in malignancies and other disorders associated with TNK1 signaling anomalies.
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