| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNIK |
| Uniprot No | Q9UKE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538 aa |
| 活性数据 | MKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSATALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW |
| 分子量 | 86.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于人TNIK蛋白的参考文献概览，涵盖其功能与疾病相关性研究：
1. **文献名称**：*Targeting TNIK signaling to inhibit colorectal cancer progression*
**作者**：Masanobu Oshima et al.
**摘要**：研究发现TNIK在结直肠癌中通过激活β-catenin/TCF复合物促进肿瘤增殖，小分子抑制剂NCB-0846可显著抑制肿瘤生长，为治疗提供新靶点。
2. **文献名称**：*Structural basis of TNIK kinase activation and its inhibition by inhibitors*
**作者**：Naoya Shindo et al.
**摘要**：通过X射线晶体学解析TNIK激酶结构，揭示其活性构象及与抑制剂的结合模式，为设计高选择性抗癌药物奠定基础。
3. **文献名称**：*TNIK is a novel regulator of neural progenitor cell differentiation*
**作者**：Yoshio Goshima et al.
**摘要**：发现TNIK通过调控MAPK通路影响神经前体细胞分化，敲低TNIP导致小鼠胚胎期神经元发育异常，提示其在神经系统疾病中的作用。
4. **文献名称**：*Recombinant TNIK purification and its role in JNK pathway activation*
**作者**：Hiroshi Takeshita et al.
**摘要**：建立重组人TNIK蛋白的高效表达纯化方法，验证其体外激活JNK信号通路的能力，为激酶活性研究提供工具。
提示：以上文献示例为模拟内容，实际研究中建议通过PubMed/Google Scholar搜索具体论文。TNIK相关研究多聚焦于癌症、神经疾病及激酶结构/抑制剂开发领域。
Traf2- and Nck-interacting kinase (TNIK), a serine/threonine kinase belonging to the germinal center kinase (GCK) family, plays critical roles in regulating cellular processes such as cytoskeletal organization, cell migration, and signal transduction. Initially identified for its interaction with Traf2 and adaptor protein Nck, TNIK has gained attention for its involvement in the Wnt/β-catenin signaling pathway, where it activates T-cell factor/lymphoid enhancer factor (TCF/LEF)-dependent transcription—a key driver of cell proliferation and differentiation. Structurally, TNIK contains an N-terminal kinase domain and a C-terminal citron homology (CNH) domain, facilitating protein-protein interactions and subcellular localization.
Dysregulation of TNIK is implicated in multiple pathologies, including colorectal cancer, where its overexpression correlates with tumor progression and poor prognosis. Recent studies also link TNIK to neurological disorders, such as Alzheimer’s disease, due to its role in synaptic plasticity. Recombinant human TNIK protein, produced via bacterial or mammalian expression systems, is widely used to study kinase activity, screen inhibitors, and elucidate structural mechanisms. Its utility in drug discovery underscores TNIK’s potential as a therapeutic target, particularly for cancers and neurodegenerative conditions. Efforts to develop TNIK-specific inhibitors highlight its growing significance in biomedical research.
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