| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMTC4 |
| Uniprot No | Q5T4D3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395 aa |
| 活性数据 | MLCKEQGITVLGLNAVFDILVIGKFNVLEIVQKVLHKDKSLENLGMLRNGGLLFRMTLLTSGGAGMLYVRWRIMGTGPPAFTEVDNPASFADSMLVRAVNYNYYYSLNAWLLLCPWWLCFDWSMGCIPLIKSISDWRVIALAALWFCLIGLICQALCSEDGHKRRILTLGLGFLVIPFLPASNLFFRVGFVVAERVLYLPSVGYCVLLTFGFGALSKHTKKKKLIAAVVLGILFINTLRCVLRSGEWRSEEQLFRSALSVCPLNAKVHYNIGKNLADKGNQTAAIRYYREAVRLNPKYVHAMNNLGNILKERNELQEAEELLSLAVQIQPDFAAAWMNLGIVQNSLKRFEAAEQSYRTAIKHRRKYPDCYYNLGRLVSAGCPVPVEGKMGYFSYL |
| 分子量 | 70.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMTC4蛋白的3篇参考文献摘要示例：
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1. **文献名称**: *TMTC4 is a novel regulator of ER-associated protein degradation in neural development*
**作者**: Lee J, et al.
**摘要**: 该研究揭示了TMTC4在内质网相关蛋白降解(ERAD)中的作用，发现其通过与其他伴侣蛋白相互作用调控错误折叠蛋白的清除，敲低TMTC4导致神经元发育异常。重组人TMTC4蛋白被用于体外验证其生化功能。
2. **文献名称**: *Functional characterization of TMTC4 variants linked to hearing loss using recombinant protein models*
**作者**: Smith A, et al.
**摘要**: 研究利用重组TMTC4蛋白分析了导致遗传性耳聋的突变体，发现特定突变破坏其跨膜结构域稳定性，影响内质网钙离子稳态，为疾病机制提供了分子层面的证据。
3. **文献名称**: *Proteomic analysis of TMTC4-interacting partners in cancer cells*
**作者**: Chen X, et al.
**摘要**: 通过重组人TMTC4蛋白的免疫共沉淀实验，鉴定出多个肿瘤相关蛋白互作网络，提示TMTC4可能通过调控Wnt信号通路参与癌症迁移与侵袭。
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*注：以上为模拟摘要，实际文献可能需要通过PubMed或Google Scholar以“TMTC4”和“recombinant protein”等关键词检索获取具体信息。如研究较为前沿，建议扩展相关关键词(如“内质网应激”“疾病名称”)进行补充检索。*
Recombinant human TMTC4 (Transmembrane and Tetratricopeptide Repeat Containing 4) protein is a synthesized form of the endogenous TMTC4. a member of the TMTC family characterized by transmembrane domains and tetratricopeptide repeat (TPR) motifs. These structural features suggest roles in protein-protein interactions, intracellular trafficking, or post-translational modifications. TMTC4 is predominantly localized to the endoplasmic reticulum (ER) and has been implicated in N-linked glycosylation, a critical process for protein folding and quality control. Dysregulation of TMTC4 is associated with ER stress responses and may contribute to pathologies such as cancer and neurodegenerative disorders.
Recombinant expression systems (e.g., *E. coli* or mammalian cells) enable high-yield production of TMTC4 for functional studies. This protein is utilized to investigate its interaction partners, enzymatic activity in glycosylation pathways, and mechanisms underlying ER-related stress. Additionally, recombinant TMTC4 serves as an antigen for antibody development or a tool for screening potential modulators of glycosylation processes. Its study offers insights into cellular stress adaptation and therapeutic targeting of diseases linked to protein misfolding or ER dysfunction. Current research continues to clarify its precise biological roles and therapeutic relevance.
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