| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ILF3 |
| Uniprot No | Q12906-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-702aa |
| 氨基酸序列 | MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWID EQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAK GLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSV DDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDR QKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWP LELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEAT DAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNE NPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPP QAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSK KTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVE AVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGS HDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANK KKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRG RGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSDFFTDCYGYHDFG SS |
| 预测分子量 | 103 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ILF3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*ILF3 coordinates RNA degradosome assembly in stressed cells*
**作者**：Smith J, Brown A
**摘要**：研究通过重组ILF3蛋白的体外表达，揭示了ILF3在细胞应激状态下调控RNA降解体复合物形成的机制，证明其通过结合特定RNA结构域促进复合物稳定性。
2. **文献名称**：*Recombinant ILF3 enhances antiviral innate immunity via RIG-I signaling*
**作者**：Zhang Y, Li X
**摘要**：利用重组ILF3蛋白进行功能实验，发现其通过增强RIG-I对病毒RNA的识别能力，激活下游干扰素通路，为抗病毒治疗提供潜在靶点。
3. **文献名称**：*Structural insights into ILF3 dimerization using recombinant protein crystallography*
**作者**：Wang Q, Tanaka M
**摘要**：通过重组人源ILF3蛋白的结晶结构分析，揭示了其双链RNA结合域的二聚化模式及对基因表达调控的结构基础。
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注：上述文献为示例，实际引用时请核对原文信息及数据库(如PubMed、Web of Science)的准确性。
**Background of ILF3 Recombinant Protein**
ILF3 (Interleukin enhancer-binding factor 3), also known as NF90/NF110 or DRBP76. is a multifunctional RNA-binding protein involved in transcriptional and post-transcriptional regulation. It belongs to the double-stranded RNA-binding protein (dsRBP) family and plays critical roles in mRNA stability, translation, and microRNA (miRNA) processing. The ILF3 gene encodes two major isoforms, NF90 and NF110. generated through alternative splicing. These isoforms share common domains, including double-stranded RNA-binding domains (dsRBDs) and a nuclear localization signal, but differ in their C-terminal regions, leading to distinct functional properties.
ILF3 interacts with various nucleic acids (DNA/RNA) and proteins, modulating processes such as viral replication, cell cycle progression, and stress responses. It is implicated in innate immunity by regulating interferon signaling and antiviral defense mechanisms. Dysregulation of ILF3 has been linked to cancers, neurodegenerative diseases, and autoimmune disorders, highlighting its therapeutic potential.
Recombinant ILF3 proteins are engineered for functional studies, typically expressed in bacterial or eukaryotic systems (e.g., *E. coli* or mammalian cells) and purified via affinity chromatography. These proteins retain native binding activity, enabling researchers to investigate ILF3’s interactions with RNA/DNA, its role in ribonucleoprotein complexes, and its regulatory mechanisms in disease contexts. Recombinant ILF3 is also utilized in drug discovery, particularly for targeting RNA-protein interactions in antiviral or anticancer therapies.
Overall, ILF3 recombinant proteins serve as essential tools for unraveling the molecular pathways governed by ILF3 and exploring its applications in biomedical research.
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