| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT9 |
| Uniprot No | Q9UHD8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-586 aa |
| 活性数据 | MKKSYSGGTR TSSGRLRRLG DSSGPALKRS FEVEEVETPN STPPRRVQTP LLRATVASST QKFQDLGVKN SEPSARHVDS LSQRSPKASL RRVELSGPKA AEPVSRRTEL SIDISSKQVE NAGAIGPSRF GLKRAEVLGH KTPEPAPRRT EITIVKPQES AHRRMEPPAS KVPEVPTAPA TDAAPKRVEI QMPKPAEAPT APSPAQTLEN SEPAPVSQLQ SRLEPKPQPP VAEATPRSQE ATEAAPSCVG DMADTPRDAG LKQAPASRNE KAPVDFGYVG IDSILEQMRR KAMKQGFEFN IMVVGQSGLG KSTLINTLFK SKISRKSVQP TSEERIPKTI EIKSITHDIE EKGVRMKLTV IDTPGFGDHI NNENCWQPIM KFINDQYEKY LQEEVNINRK KRIPDTRVHC CLYFIPATGH SLRPLDIEFM KRLSKVVNIV PVIAKADTLT LEERVHFKQR ITADLLSNGI DVYPQKEFDE DSEDRLVNEK FREMIPFAVV GSDHEYQVNG KRILGRKTKW GTIEVENTTH CEFAYLRDLL IRTHMQNIKD ITSSIHFEAY RVKRLNEGSS AMANGMEEKE PEAPEM |
| 分子量 | 65.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于研究背景模拟生成的重组人SEPT9蛋白相关文献示例(仅供学术参考，具体文献请通过数据库核实)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant Human SEPT9 in Cancer Cell Lines*
**作者**: Li, X. et al.
**摘要**: 研究报道了通过大肠杆菌系统成功表达并纯化重组人SEPT9蛋白，并证实其在体外促进肿瘤细胞迁移，提示SEPT9可能在癌症转移中发挥作用。
2. **文献名称**: *Structural Insights into SEPT9 Filament Formation Using Recombinant Protein*
**作者**: Garcia, M. & Smith, R.
**摘要**: 利用重组SEPT9蛋白进行冷冻电镜分析，揭示了其多聚体组装机制，为septin家族在细胞骨架中的功能提供结构基础。
3. **文献名称**: *SEPT9 Methylation and Protein Detection: Implications for Colorectal Cancer Screening*
**作者**: Wang, Y. et al.
**摘要**: 开发基于重组SEPT9蛋白的抗体检测方法，结合DNA甲基化分析，验证其作为结直肠癌无创诊断生物标志物的潜力。
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**注意**：以上文献信息为模拟生成，如需真实参考文献，建议通过**PubMed**或**Web of Science**搜索关键词 "recombinant SEPT9" 或 "SEPT9 protein" 获取最新研究。实际研究中需注意文献的权威性和实验可重复性。
**Background of Recombinant Human SEPT9 Protein**
Septin 9 (SEPT9), a member of the conserved septin family of GTP-binding proteins, plays critical roles in cytoskeletal organization, cell division, membrane dynamics, and vesicle trafficking. It exists as multiple isoforms due to alternative splicing, with its longest isoform containing a conserved N-terminal GTPase domain, a central polybasic region, and a C-terminal coiled-coil domain. SEPT9 interacts with other septins to form hetero-oligomeric complexes, which assemble into higher-order structures to regulate cellular processes.
Dysregulation of SEPT9 is implicated in diverse pathologies, including cancer, neurodegenerative diseases, and infections. Notably, hypermethylation of the *SEPT9* gene promoter is a validated biomarker for non-invasive colorectal cancer detection. In cancer, SEPT9 overexpression promotes tumorigenesis by enhancing cell proliferation, invasion, and resistance to apoptosis, while its loss may disrupt mitotic fidelity.
Recombinant human SEPT9 protein, produced via bacterial or mammalian expression systems, enables biochemical and functional studies. Its purified form facilitates in vitro investigations into GTPase activity, filament assembly, protein interactions (e.g., with actin or microtubules), and the impact of disease-associated mutations. Researchers also utilize it to develop targeted therapies or diagnostic tools, given its clinical relevance. Despite progress, the precise molecular mechanisms and isoform-specific functions of SEPT9 remain under exploration, highlighting its importance in both basic and translational research.
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