| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT14 |
| Uniprot No | A6QQL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432 aa |
| 活性数据 | MAEKPMCPST QIPDDEDTQK EDNIRCLNML GHFGFDCLAH QLVDKSVQQG FFFNILCVGE TGIGKSTLID TLFNTSLKDK KSSHFYASVG LKIQTYELQE NNVQLKLTVV KTVGYGDQIN KEASYQPIVD YLDAQFESYL QEELKIKRSL GNYRDSRVHV CLYFISPTGH SLKSLDILTM KNIDSKVNII PVIAKADAIS KSDLQTFKCA IMNELISNGI QMYQFPTDNE TSTHMNSSMN GLLPFAVVGS TEEVKVGKRT VRGRQYPWGI LQVENENHCD FVKLRDMLLC TNREDLKEQT HTRHYERYRR NRLHMMGFTD MGPNNQPVSF QEIYEAKRQE LLEQCQREEE ELKHKFMQRV KEKETAFKEA EKELQDKFEH LKKVQQEETM RLEEERRQLE EEILELCKMK ASSGTLQSQV CTSVKKDKER KK |
| 分子量 | 50.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SEPT14蛋白的示例参考文献(注：部分信息为示例性概括，实际文献需另行检索确认)：
1. **"Functional analysis of recombinant human SEPT14 in spermatogenesis"**
*作者：Kuo, Y.C., et al. (2015)*
**摘要**：研究通过在大肠杆菌中重组表达SEPT14蛋白，验证其与精子形成相关的相互作用网络，发现其在精子头部形态发生中起关键作用，突变体导致精子畸形和不育表型。
2. **"Structural characterization of recombinant SEPT14 using X-ray crystallography"**
*作者：Hall, T., & Johnson, R. (2018)*
**摘要**：报道SEPT14蛋白的重组表达及纯化方法，通过晶体结构解析揭示其GTP酶结构域特征，为Septin家族蛋白的多聚体组装机制提供依据。
3. **"SEPT14 mutations impair neurodevelopment via disrupted microtubule dynamics"**
*作者：Suzuki, M., et al. (2020)*
**摘要**：利用昆虫细胞系统重组表达SEPT14.结合CRISPR敲除模型，阐明其突变通过干扰微管稳定性影响神经元迁移，提示与神经系统发育障碍的关联。
4. **"Recombinant SEPT14 as a biomarker for male infertility screening"**
*作者：Li, W., et al. (2017)*
**摘要**：开发基于重组SEPT14的抗体检测技术，分析不育患者样本中SEPT14表达水平，证实其蛋白缺失可作为诊断少精症的潜在标志物。
(以上文献为示例性内容，实际引用请通过学术数据库核实。)
**Background of Recombinant Human SEPT14 Protein**
The septin family comprises a conserved group of GTP-binding proteins involved in diverse cellular processes, including cytoskeletal organization, cell division, vesicle trafficking, and membrane remodeling. SEPT14 (septin 14), a member of this family, plays critical roles in spermiogenesis, neuronal development, and cilia function. It is expressed predominantly in the testes and brain, where it contributes to the structural and functional regulation of microtubule networks and actin filaments. SEPT14 interacts with other septins to form heteropolymeric filaments, which are essential for maintaining cellular polarity and compartmentalization.
Recombinant human SEPT14 protein is engineered through molecular cloning and expressed in bacterial or eukaryotic systems, enabling high-purity production for functional studies. Its recombinant form allows researchers to investigate molecular mechanisms underlying SEPT14-associated pathologies, such as male infertility, neurodegenerative disorders, and ciliopathies. Structural analyses of recombinant SEPT14 have revealed conserved GTPase domains and unique C-terminal extensions critical for protein-protein interactions and post-translational modifications. Current research focuses on deciphering its role in cellular dynamics, disease models, and potential therapeutic targeting. The availability of recombinant SEPT14 facilitates biochemical assays, antibody development, and screening for modulators of septin-mediated pathways.
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