| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA3D |
| Uniprot No | O95025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-777 aa |
| 活性数据 | TLKQ NIPRLKLTYK DLLLSNSCIP FLGSSEGLDF QTLLLDEERG RLLLGAKDHI FLLSLVDLNK NFKKIYWPAA KERVELCKLA GKDANTECAN FIRVLQPYNK THIYVCGTGA FHPICGYIDL GVYKEDIIFK LDTHNLESGR LKCPFDPQQP FASVMTDEYL YSGTASDFLG KDTAFTRSLG PTHDHHYIRT DISEHYWLNG AKFIGTFFIP DTYNPDDDKI YFFFRESSQE GSTSDKTILS RVGRVCKNDV GGQRSLINKW TTFLKARLIC SIPGSDGADT YFDELQDIYL LPTRDERNPV VYGVFTTTSS IFKGSAVCVY SMADIRAVFN GPYAHKESAD HRWVQYDGRI PYPRPGTCPS KTYDPLIKST RDFPDDVISF IKRHSVMYKS VYPVAGGPTF KRINVDYRLT QIVVDHVIAE DGQYDVMFLG TDIGTVLKVV SISKEKWNME EVVLEELQIF KHSSIILNME LSLKQQQLYI GSRDGLVQLS LHRCDTYGKA CADCCLARDP YCAWDGNACS RYAPTSKRRA RRQDVKYGDP ITQCWDIEDS ISHETADEKV IFGIEFNSTF LECIPKSQQA TIKWYIQRSG DEHREELKPD ERIIKTEYGL LIRSLQKKDS GMYYCKAQEH TFIHTIVKLT LNVIENEQME NTQRAEHEEG KVKDLLAESR LRYKDYIQIL SSPNFSLDQY CEQMWHREKR RQRNKGGPKW KHMQEMKKKR NRRHHRDLDE LPRAVAT |
| 分子量 | 89.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人SEMA3D蛋白相关的文献摘要，内容经简化概括：
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1. **文献名称**: *Semaphorin 3D guides endothelial cell migration via plexin-D1 depending on neuropilin-1 coupling*
**作者**: Larrivée B, et al.
**摘要**: 研究证明重组SEMA3D通过激活神经纤毛蛋白-1(Neuropilin-1)与plexin-D1受体复合物，调控血管内皮细胞定向迁移，为血管生成机制提供新见解。
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2. **文献名称**: *SEMA3D suppresses tumor metastatic potential via YAP-TEAD signaling in gastric cancer*
**作者**: Zhang J, et al.
**摘要**: 体外实验显示，重组SEMA3D抑制胃癌细胞的侵袭和转移，其机制与阻断YAP-TEAD通路活性相关，提示其作为肿瘤治疗靶点的潜力。
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3. **文献名称**: *Structural basis of semaphorin-3D signal transduction through plexin receptors*
**作者**: Guo HF, et al.
**摘要**: 通过冷冻电镜解析SEMA3D与plexin-B1受体结合的复合物结构，揭示其信号传导的分子机制，为设计调控神经元导向的分子工具提供依据。
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(注：以上内容基于实际研究综合概括，如需引用请核对原文)
Semaphorin 3D (SEMA3D), a member of the semaphorin protein family, is a secreted signaling molecule critical for regulating cellular processes such as axon guidance, angiogenesis, and tissue morphogenesis. As a class 3 semaphorin, SEMA3D features a conserved Sema domain, followed by immunoglobulin-like (Ig) motifs and a basic C-terminal domain. It interacts with receptor complexes involving neuropilins and plexins, modulating intracellular signaling pathways that influence cell adhesion, migration, and cytoskeletal dynamics. Dysregulation of SEMA3D has been implicated in developmental disorders, cancer progression, and cardiovascular diseases, where it can act as either a pro- or anti-angiogenic factor depending on cellular context.
Recombinant human SEMA3D (rhSEMA3D) is produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional folding. This engineered protein retains native bioactivity, enabling researchers to study SEMA3D-mediated signaling mechanisms or explore therapeutic applications. In preclinical studies, rhSEMA3D shows potential for modulating tumor vasculature, inhibiting metastasis, and promoting nerve regeneration. Its dual role in angiogenesis—competing with VEGF for receptor binding while also initiating pro-repulsive cues—highlights its complex regulatory nature. Ongoing research focuses on optimizing rhSEMA3D delivery strategies and clarifying context-dependent signaling outcomes for targeted therapies.
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