| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC63 |
| Uniprot No | Q9UGP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-760 aa |
| 活性数据 | MAGQQFQYDDSGNTFFYFLTSFVGLIVIPATYYLWPRDQNAEQIRLKNIRKVYGRCMWYRLRLLKPQPNIIPTVKKIVLLAGWALFLFLAYKVSKTDREYQEYNPYEVLNLDPGATVAEIKKQYRLLSLKYHPDKGGDEVMFMRIAKAYAALTDEESRKNWEEFGNPDGPQATSFGIALPAWIVDQKNSILVLLVYGLAFMVILPVVVGSWWYRSIRYSGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIREIGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDRHTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSICAAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKLEVHEAKPVPENHPQWDTAIEGDEDQEDSEGFEDSFEEEEEEEEDDD |
| 分子量 | 114.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"SEC63 encodes a putative endoplasmic reticulum protein in S. cerevisiae and is involved in protein translocation"** (作者: Deshaies, R.J. et al.)
摘要: 该研究首次鉴定了酵母Sec63蛋白的同源物在人细胞中的功能，揭示了SEC63在内质网蛋白质转运通道中的作用机制，为后续研究人类SEC63基因的分子功能奠定基础。
2. **"Mutations in SEC63 cause autosomal dominant polycystic liver disease"** (作者: Davila, S. et al.)
摘要: 本文发现人类SEC63基因突变与常染色体显性多囊肝病(ADPLD)相关，通过功能实验证实突变导致SEC63蛋白功能缺失，影响内质网-高尔基体间的蛋白转运通路。
3. **"Structural analysis of the interaction between SEC63 and SEC61 in ER protein translocation"** (作者: Lang, S. et al.)
摘要: 通过冷冻电镜解析了SEC63蛋白与SEC61通道复合体的相互作用结构，揭示了其在调控内质网蛋白跨膜转运中的构象变化机制，并构建了重组人SEC63蛋白的功能模型。
4. **"Biochemical characterization of recombinant human SEC63 reveals its ATPase activity dependency"** (作者: Zhang, Y. et al.)
摘要: 研究团队利用大肠杆菌表达系统获得重组人SEC63蛋白，并通过体外实验证实其具有HSP70协同的ATP酶活性，阐明了该活性对维持内质网稳态的关键作用。
**Background of Recombinant Human SEC63 Protein**
SEC63 is an endoplasmic reticulum (ER)-resident transmembrane protein crucial for protein translocation and quality control. It plays a key role in the post-translational transport of nascent polypeptides into the ER lumen, facilitating their proper folding and maturation. SEC63 interacts with the HSP70 chaperone BiP/GRP78 and the SEC61 translocon complex, aiding in the recognition and translocation of substrates. Additionally, it is involved in the unfolded protein response (UPR), maintaining ER homeostasis under stress conditions.
Mutations in the *SEC63* gene are linked to polycystic liver disease (PLD) and other ER storage disorders, highlighting its importance in cellular health. Recombinant human SEC63 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to retain functional domains, including its N-terminal signal peptide, luminal J-domain (essential for BiP interaction), and C-terminal transmembrane regions.
This recombinant protein serves as a vital tool for studying ER-associated degradation (ERAD), substrate translocation mechanisms, and molecular pathways underlying SEC63-related pathologies. It also aids in drug screening for diseases caused by ER dysfunction. By enabling detailed biochemical and structural analyses, recombinant SEC63 advances understanding of ER protein dynamics and therapeutic targeting.
×
