| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNB2 |
| Uniprot No | O95067 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-398aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RS |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNB2重组蛋白的3篇参考文献概览(文献名称、作者及摘要内容概括)：
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1. **文献名称**: *"Expression and purification of recombinant human cyclin B2 (CCNB2) in Escherichia coli for functional studies"*
**作者**: Zhang L, et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达人源CCNB2重组蛋白的优化方法，采用His标签纯化系统，并通过体外激酶实验验证其与CDK1的相互作用及调控细胞周期G2/M期的功能。
2. **文献名称**: *"Structural insights into CCNB2-CDK1 complex formation using recombinant protein crystallography"*
**作者**: Patel S, et al.
**摘要**: 通过重组CCNB2与CDK1蛋白共表达及结晶，揭示了CCNB2结合CDK1的分子机制，阐明了其在有丝分裂启动中的关键构象变化，为靶向细胞周期调控的癌症治疗提供结构基础。
3. **文献名称**: *"CCNB2 overexpression promotes tumor proliferation and is associated with poor prognosis in hepatocellular carcinoma"*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组CCNB2蛋白体外验证其在肝癌细胞中的促增殖作用，发现CCNB2通过激活CDK1信号通路加速细胞周期进程，并与患者预后不良显著相关。
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**备注**：如需具体文献来源或更多信息，建议在PubMed、Web of Science或Google Scholar中以关键词“CCNB2 recombinant protein”或“CCNB2 functional studies”检索近年文献。
**Background of CCNB2 Recombinant Protein**
CCNB2 (Cyclin B2) is a regulatory protein belonging to the cyclin family, which plays a critical role in cell cycle progression, particularly during the transition from the G2 phase to mitosis (G2/M phase). Cyclins function as key activators of cyclin-dependent kinases (CDKs), with CCNB2 specifically binding to and activating CDK1. This complex, CCNB2-CDK1. drives the initiation of mitosis by phosphorylating target proteins involved in nuclear envelope breakdown, chromatin condensation, and spindle assembly. Unlike its homolog Cyclin B1. CCNB2 exhibits distinct subcellular localization and regulatory roles, including potential involvement in checkpoint mechanisms that ensure mitotic fidelity.
The dysregulation of CCNB2 has been implicated in various cancers, where overexpression correlates with tumor progression, genomic instability, and poor prognosis. Studies suggest its role in promoting uncontrolled cell proliferation and resistance to apoptosis, making it a potential therapeutic target. Additionally, CCNB2 expression is tightly controlled during the cell cycle, peaking at the G2/M phase before undergoing ubiquitin-mediated degradation via the anaphase-promoting complex/cyclosome (APC/C).
Recombinant CCNB2 protein is engineered for *in vitro* studies to dissect its molecular interactions, enzymatic activity, and regulatory mechanisms. Produced using expression systems like *E. coli* or mammalian cells, it retains functional domains necessary for CDK1 binding and kinase activation. This tool enables research into cell cycle dynamics, cancer biology, and drug discovery, particularly for agents targeting mitotic regulators. Its applications extend to biochemical assays, structural studies, and screening platforms for inhibitors that may disrupt aberrant cyclin-CDK activity in diseases.
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