| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSG1 |
| Uniprot No | Q9H089 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-658aa |
| 活性数据 | MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAGTEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDSQSNTTEFGHSSFDQAEISHSESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRKTPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINIITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM |
| 分子量 | 98.78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LSG1蛋白的参考文献示例(内容虚构，供参考)：
1. **文献名称**: *Structural and Functional Analysis of Human LSG1 Reveals its Role in Ribosome Biogenesis*
**作者**: Müller, A. et al.
**摘要**: 本研究解析了重组人LSG1蛋白的晶体结构，揭示了其GTP酶结构域的关键作用。通过体外实验证明，LSG1通过水解GTP促进核糖体60S亚基的成熟，缺失LSG1导致细胞内核糖体组装异常和增殖缺陷。
2. **文献名称**: *Recombinant LSG1 as a Therapeutic Target in Ribosomopathies*
**作者**: Chen, L. & Park, J.H.
**摘要**: 研究发现，LSG1突变与先天性核糖体病相关。通过表达纯化重组人LSG1蛋白，验证了其突变体(如R257Q)的GTP酶活性丧失，导致细胞周期停滞，为靶向治疗提供了潜在策略。
3. **文献名称**: *Development of a High-Yield Expression System for Human LSG1 in E. coli*
**作者**: Gupta, S. et al.
**摘要**: 提出一种基于大肠杆菌的重组人LSG1蛋白高效表达纯化方案，并验证其酶动力学特性。该体系为大规模制备LSG1以研究其分子机制奠定了基础。
4. **文献名称**: *LSG1 Knockdown Disrupts Nucleolar-Cytoplasmic Transport of Pre-60S Ribosomes*
**作者**: Silva, R. et al.
**摘要**: 利用siRNA敲低LSG1.结合活细胞成像技术，揭示LSG1在核糖体前体从核仁向细胞质转运中的必要性，重组LSG1可部分挽救因基因缺失导致的转运缺陷。
(注：以上内容为模拟生成，实际文献需通过学术数据库检索核实。)
Recombinant human LSG1 protein is a genetically engineered version of the large subunit GTPase 1 (LSG1), a conserved eukaryotic enzyme critical for ribosome biogenesis. LSG1 plays a key role in the maturation of the 60S ribosomal subunit, particularly in the final steps of nucleolar-cytoplasmic transport and assembly. It functions as a GTPase, hydrolyzing GTP to regulate conformational changes essential for releasing ribosomal assembly factors and ensuring proper ribosome function. Dysregulation of LSG1 has been implicated in cellular stress responses, cancer progression, and neurodegenerative disorders, highlighting its importance in maintaining proteostasis and cell viability.
The recombinant form is typically produced using expression systems like E. coli or mammalian cell cultures, enabling controlled studies of its structure, interactions, and biochemical activities. Researchers utilize recombinant LSG1 to investigate its GTPase mechanism, its partnership with cofactors like NSA2 (Nop-seven-associated 2), and its role in ribosome quality control pathways. Recent studies also explore its potential as a therapeutic target, given its overexpression in certain cancers and involvement in stress granule dynamics under pathological conditions. Structural analyses reveal conserved GTP-binding domains and unique helical regions that mediate protein-RNA interactions. As a research tool, recombinant LSG1 facilitates mechanistic insights into ribosome assembly diseases and drug discovery efforts targeting translational regulation.
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