| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRWD1 |
| Uniprot No | Q9UFC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-647aa |
| 活性数据 | MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM |
| 分子量 | 97.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRWD1蛋白研究的参考文献示例(文献为模拟内容，建议通过学术数据库查询真实文献)：
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1. **文献名称**：LRWD1 interacts with the origin recognition complex to regulate heterochromatin formation in spermatogenesis
**作者**：Zhang Y. et al.
**摘要**：该研究阐明了人LRWD1蛋白与ORC(原点识别复合体)的相互作用，发现重组人LRWD1通过结合异染色质区域，调控精子发生过程中的染色质沉默和基因组稳定性。
2. **文献名称**：Structural insights into LRWD1-ORC interaction by cryo-EM
**作者**：Li X. et al.
**摘要**：利用冷冻电镜解析了重组人LRWD1蛋白与ORC亚基的复合体结构，揭示了LRWD1在DNA复制起始阶段通过稳定ORC结合促进染色质组装的作用机制。
3. **文献名称**：CRISPR screen identifies LRWD1 as a key regulator of DNA replication fidelity
**作者**：Chen L. et al.
**摘要**：通过功能筛选发现，重组人LRWD1蛋白通过调节复制叉进程和异染色质维持，影响人类细胞中DNA复制的保真性，并与癌症基因组不稳定性相关。
4. **文献名称**：Recombinant LRWD1 expression and purification for in vitro chromatin assembly assays
**作者**：Wang R. et al.
**摘要**：报道了重组人LRWD1蛋白在大肠杆菌中的高效表达与纯化方法，并证明其在体外染色质重建实验中对核小体定位的调控功能。
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建议通过 **PubMed/Google Scholar** 以关键词“LRWD1 recombinant”或“LRWD1 ORC complex”检索最新文献，获取具体研究数据。
Recombinant human LRWD1 (leucine-rich repeat and WD repeat domain-containing protein 1) is a protein encoded by the *LRWD1* gene, which plays a role in chromatin organization and transcriptional regulation. LRWD1 contains two conserved structural motifs: leucine-rich repeats (LRRs) involved in protein-protein interactions and WD40 repeats that facilitate scaffolding within multiprotein complexes. It is predominantly expressed in germ cells and somatic tissues, with critical functions in maintaining heterochromatin stability, particularly at pericentromeric regions. Studies suggest its interaction with the origin recognition complex (ORC), linking it to DNA replication licensing and cell cycle progression.
Interest in recombinant LRWD1 stems from its role in regulating epigenetic states, including histone modification patterns and telomere integrity. Dysregulation of LRWD1 has been implicated in male infertility due to its importance in spermatogenesis, as well as in cancer progression through genomic instability mechanisms. Recombinant production of LRWD1 (typically in *E. coli* or mammalian systems) enables biochemical studies, such as binding assays with chromatin components or ORC subunits, and structural analyses to elucidate its molecular mechanisms. Current research focuses on its dual roles in chromatin dynamics and germ cell development, offering potential insights into reproductive health and epigenetic therapies.
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