| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LETM2 |
| Uniprot No | Q2VYF4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 活性数据 | MKNYESKKYSDPSQPGNTVLHPGTRLIQKLHTSTCWLQEVPGKPQLEQATKHPQVTSPQATKETGMEIKEGKQSYRQKIMDELKYYYNGFYLLWIDAKVAARMVWRLLHGQVLTRRERRREEKQKKKMAVKLELAKFLQETMTEMARRNRAKMGDASTQLSSYVKQVQTGHKPSTKEIVRFSKLFEDQLALEHLDRPQLVALCKLLELQTFGTNNLLRFQLLMKLKSIKADDEIIAKEGVTALSVSELQAACRARGMRSLGLTEEQLRQQLTEWQDLHLKENVPPSLLLLSRTFYLIDVKPKPIEIPLSGEAPKTDILVELPTFTESKENMVDLAPQLKGTKDEDFIQPPPVTSSPITPSTPISLPKGPITSSEEPTLQAKSQMTAQNSKASSKGA |
| 分子量 | 71.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LETM2(leucine zipper-EF-hand-containing transmembrane 2)蛋白的模拟参考文献示例，供参考(建议通过PubMed或Web of Science核实真实文献信息)：
1. **文献名称**：*"Expression and functional analysis of recombinant human LETM2 in mitochondrial ion transport"*
**作者**：Chen et al.
**摘要**：研究利用哺乳动物细胞系统表达重组人LETM2蛋白，发现其定位于线粒体内膜，并通过调控钙离子转运影响线粒体能量代谢，提示其在细胞凋亡中的潜在作用。
2. **文献名称**：*"LETM2 promotes cancer cell migration by interacting with mTORC1 signaling pathway"*
**作者**：Zhang Y, Wang L.
**摘要**：通过原核表达系统纯化重组人LETM2.结合细胞实验证明LETM2通过激活mTORC1通路增强肺癌细胞的迁移和侵袭能力，为癌症靶向治疗提供依据。
3. **文献名称**：*"Structural characterization of recombinant human LETM2 and its role in Warburg effect regulation"*
**作者**：Kim S et al.
**摘要**：报道利用大肠杆菌系统高效表达可溶性重组人LETM2蛋白，晶体结构解析显示其EF-hand结构域关键位点突变会破坏与ATP合酶的结合，影响糖酵解途径。
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**注**：以上为模拟文献，实际研究中建议通过关键词 **"recombinant human LETM2"** 或 **"LETM2 protein function"** 在学术数据库中检索最新论文(如NCBI PubMed、ScienceDirect)。真实文献可能更关注LETM2与神经退行性疾病、线粒体膜电位调控等领域。
**Background of Recombinant Human LETM2 Protein**
Leucine zipper-EF-hand containing transmembrane protein 2 (LETM2) is a mitochondrial inner membrane protein encoded by the *LETM2* gene, belonging to the LETM1/2 family. Structurally, it features conserved EF-hand domains (implicated in calcium binding) and a leucine zipper motif for protein-protein interactions. LETM2 plays critical roles in mitochondrial homeostasis, including ion transport (particularly Ca²⁺ regulation), energy metabolism, and apoptosis. Dysregulation of LETM2 is linked to cellular stress responses and tumorigenesis. Studies suggest its overexpression in certain cancers (e.g., breast, lung) correlates with enhanced proliferation, metastasis, and chemoresistance, likely via interactions with mitochondrial networks and Wnt/β-catenin signaling pathways.
Recombinant human LETM2 protein, produced via expression systems like *E. coli* or mammalian cells, retains functional epitopes for biochemical and cellular studies. It enables exploration of LETM2’s molecular mechanisms, screening for inhibitors, and antibody development for diagnostic or therapeutic use. Research on recombinant LETM2 aims to clarify its dual roles in mitochondrial physiology and cancer progression, offering potential as a biomarker or target for anticancer therapies. Current challenges involve resolving its precise calcium-regulatory mechanisms and validating its clinical relevance in diverse malignancies.
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