| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LEO1 |
| Uniprot No | Q8WVC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-666aa |
| 活性数据 | MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEGASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKANSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSEDEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTSDEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD |
| 分子量 | 101.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人LEO1蛋白相关的参考文献及其摘要概括(示例为虚构，仅供参考)：
1. **文献名称**：*Structure and Function of Human LEO1 in Transcriptional Regulation*
**作者**：Kim et al.
**摘要**：本研究解析了重组人LEO1蛋白的晶体结构，揭示其与PAF复合体其他亚基的相互作用机制，并证明其在RNA聚合酶Ⅱ介导的转录延伸中的关键作用。
2. **文献名称**：*LEO1 Regulates Histone Modifications and Cancer Cell Proliferation*
**作者**：Zhang et al.
**摘要**：通过重组表达LEO1蛋白，作者发现其与组蛋白H2B泛素化修饰相关，并在肺癌细胞中调控细胞周期相关基因的表达，提示其作为肿瘤治疗靶点的潜力。
3. **文献名称**：*Recombinant LEO1 Facilitates Chromatin Remodeling In Vitro*
**作者**：Thompson et al.
**摘要**：开发了重组人LEO1蛋白的高效纯化方法，并在体外实验中证明其与染色质重塑复合体协同作用，影响基因启动子区域的可及性。
4. **文献名称**：*Functional Analysis of LEO1 in Stem Cell Differentiation*
**作者**：Wang et al.
**摘要**：利用重组LEO1蛋白进行功能实验，发现其通过调控多能性基因(如OCT4)的表观遗传修饰，影响胚胎干细胞的自我更新与分化。
注：以上文献为模拟示例，实际研究需通过数据库(如PubMed、Web of Science)检索。
The human LEO1 (Leucine-Embedded Open Reading Frame 1) protein is a critical subunit of the PAF1 complex (RNA polymerase II-associated factor 1 complex), which plays a central role in transcriptional regulation and chromatin modification. Initially identified in yeast, LEO1 is evolutionarily conserved and essential for coordinating transcription elongation, mRNA processing, and histone modification. In humans, the PAF1 complex, comprising LEO1. PAF1. CTR9. CDC73. and WDR61. associates with RNA polymerase II to facilitate transcriptional activation or repression. LEO1 specifically contributes to the complex’s stability and recruitment to chromatin, where it regulates histone H2B ubiquitination and H3K4 methylation—key epigenetic marks linked to gene expression.
Recombinant human LEO1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. Its production enables researchers to dissect molecular mechanisms underlying transcriptional regulation, chromatin dynamics, and crosstalk between transcription and DNA repair. Studies using recombinant LEO1 have highlighted its role in embryonic development, stem cell maintenance, and oncogenesis, as dysregulation of PAF1 complex components is associated with cancers and developmental disorders.
Research on recombinant LEO1 also aids in understanding diseases linked to epigenetic or transcriptional dysfunction. Its biochemical characterization provides insights into interactions with other regulatory proteins, offering potential therapeutic targets. Overall, LEO1 serves as a vital tool for exploring gene expression control and its pathological implications.
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