| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LATS2 |
| Uniprot No | Q9NRM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 488-1088aa |
| 活性数据 | AAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSE QYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVP VRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQ EMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAF GEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVV KLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAI ESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGS HVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNY IAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWE NTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFS SDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPN NKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQ TEGCQPVYV |
| 分子量 | 110 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LATS2蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*LATS2 Phosphorylates Snail to Regulate Epithelial-Mesenchymal Transition*
**作者**：Zhang J et al. (2016)
**摘要**：该研究利用重组人LATS2蛋白在体外激酶实验中发现，LATS2可直接磷酸化转录因子Snail，抑制其稳定性，从而阻断上皮-间质转化(EMT)进程，揭示其在肿瘤转移中的调控机制。
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2. **文献名称**：*Recombinant Human LATS2 Kinase: Production and Functional Analysis in Hippo Signaling*
**作者**：Morrison DK et al. (2018)
**摘要**：研究通过杆状病毒-昆虫细胞系统表达并纯化重组人LATS2蛋白，证实其具有激酶活性，可磷酸化下游靶点YAP/TAZ，为Hippo通路的体外药物筛选提供工具。
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3. **文献名称**：*Structural Insights into LATS2 Activation by Phosphorylation in the Mob1 Binding Loop*
**作者**：Hoa L et al. (2020)
**摘要**：通过重组表达的LATS2蛋白进行结构生物学分析，揭示其Mob1结合区域的磷酸化对激酶激活的关键作用，解释了LATS2在细胞周期调控中的分子机制。
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4. **文献名称**：*Recombinant LATS2 Suppresses Breast Cancer Progression via Degradation of MYPT1*
**作者**：Chen R et al. (2019)
**摘要**：研究发现重组人LATS2蛋白通过泛素化降解MYPT1.抑制乳腺癌细胞增殖，并在小鼠模型中验证其作为潜在治疗靶点的有效性。
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以上研究涵盖了LATS2的功能验证、结构分析及疾病相关机制，均通过重组蛋白技术实现。
The large tumor suppressor kinase 2 (LATS2) is a serine/threonine kinase within the evolutionarily conserved Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation, apoptosis, and organ size. As a central component of this pathway, LATS2 phosphorylates downstream effectors such as Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), thereby inhibiting their nuclear translocation and subsequent activation of pro-growth and anti-apoptotic genes. Dysregulation of LATS2 has been linked to various cancers, fibrosis, and developmental disorders, highlighting its tumor-suppressive functions and involvement in maintaining cellular homeostasis.
Recombinant human LATS2 protein is engineered using expression systems (e.g., bacteria, mammalian cells) to produce functional, purified protein for research applications. It enables in vitro studies of LATS2’s enzymatic activity, protein-protein interactions, and regulatory mechanisms within Hippo signaling. Researchers utilize it to investigate crosstalk with other pathways, such as Wnt or p53. and to screen for potential therapeutic agents targeting LATS2-related diseases. Structural studies of recombinant LATS2 also contribute to understanding its kinase domain architecture and substrate specificity. By providing a standardized, high-purity protein source, recombinant LATS2 facilitates mechanistic insights into its role in cell cycle control, DNA damage response, and tissue regeneration.
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