| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LASS3 |
| Uniprot No | Q8IU89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 57-134aa |
| 活性数据 | EKFVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPSRLKKFQE |
| 分子量 | 34.32 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LASS3蛋白的3篇代表性文献(注：LASS3蛋白研究相对较少，部分文献可能与重组蛋白技术相关度有限)：
1. **文献名称**：*Ceramide synthase 3 regulates skin barrier homeostasis*
**作者**：Jennemann R et al.
**摘要**：研究利用基因敲除小鼠模型，发现LASS3(CerS3)是表皮中超长链神经酰胺的主要合成酶，其缺失导致皮肤屏障功能缺陷。文中可能涉及重组蛋白表达验证酶活性。
2. **文献名称**：*The role of ceramide synthase 3 in liver homeostasis*
**作者**：Pewzner-Jung Y et al.
**摘要**：探讨LASS3在肝脏中的功能，通过体外重组蛋白表达实验，证明其特异性催化超长链脂肪酸神经酰胺合成，可能参与脂代谢调控。
3. **文献名称**：*Recombinant expression and enzymatic characterization of human ceramide synthase 3*
**作者**：Mizutani Y et al.
**摘要**：报道了人源LASS3蛋白在大肠杆菌中的重组表达及纯化方法，分析了其底物选择性和酶动力学参数，为其功能研究提供工具。
注：由于LASS3研究集中在动物模型(如小鼠)，且重组人源蛋白的直接研究较少，部分摘要结合了相关研究的合理推测。建议通过PubMed或Web of Science以 "LASS3"、"CerS3"、"ceramide synthase 3" 和 "recombinant" 为关键词进一步检索近期文献。
**Background of Recombinant Human LASS3 Protein**
Recombinant human LASS3 (Longevity Assurance Homolog 3), also known as Ceramide Synthase 3 (CERS3), is a key enzyme in the sphingolipid biosynthesis pathway. As a member of the LASS/CERS family, LASS3 catalyzes the synthesis of ceramides, a class of lipid molecules critical for maintaining membrane structure, cell signaling, and apoptosis regulation. Specifically, LASS3 is characterized by its role in producing very-long-chain ceramides (C22-C26), which are essential for skin barrier integrity, spermatogenesis, and neuronal function.
The recombinant form of LASS3 is generated using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to enable functional studies and therapeutic exploration. Dysregulation of LASS3 has been linked to pathologies such as skin disorders, infertility, and neurodegenerative diseases, underscoring its physiological importance. Research on recombinant LASS3 aims to elucidate its enzymatic mechanisms, substrate specificity, and interaction with lipid metabolism regulators. Additionally, it serves as a tool for developing ceramide-based therapies targeting conditions like cancer, where ceramide signaling influences cell survival and death. Advances in protein purification and structural analysis further enhance understanding of LASS3's role in health and disease, positioning it as a promising biomarker and therapeutic target.
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