关键信息
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基因名
Angiopoietin-1
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简介
The Angiopoietin-1 Protein is a secreted glycoprotein that is a key ligand in the vascular tyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells. Angiopoietin-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Angiopoietin-1 protein, expressed by HEK293 , with N-His labeled tag.
- 应用
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别名
AGP1; AGPT; ANG-1; Angiopoietin-1; ANGPT1
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种属
Cynomolgus
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表达系统
HEK293
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标签
N-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
XP_005563980
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表达区间
R276-F497
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氨基酸序列
REEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF
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蛋白长度
Partial
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分子量
30-34 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Angiopoietin-1 (Ang-1) is a crucial protein that plays a significant role in angiogenesis, the process of new blood vessel formation, which is essential for maintaining vascular stability and regulating inflammation. It is part of the Angiopoietin/Tie signaling pathway, where Ang-1 binds to its receptor Tie-2, facilitating vascular maturation and stabilization. Research has highlighted Ang-1's potential in therapeutic applications, particularly in conditions such as cardiovascular diseases, cancer, and wound healing, where angiogenesis is often disrupted. Recombinant Angiopoietin-1 proteins are being studied for their ability to enhance endothelial cell survival, proliferation, and migration, ultimately promoting healthy blood vessel formation. Additionally, Ang-1's anti-inflammatory properties are of interest, as they could mitigate tissue injury and promote repair mechanisms. As scientists explore the molecular mechanisms underlying Ang-1's functions, recombinant protein technology offers opportunities for developing novel treatments that harness the protective effects of Ang-1, making it a promising candidate in regenerative medicine and vascular therapy.
