Recombinant Human KCNH7 Protein
- 中文名： 重组人KCNH7蛋白
- 别 名： KCNH7; ERG3Potassium voltage-gated channel subfamily H member 7; Ether-a-go-go-related gene potassium channel 3; ERG-3; Eag-related protein 3; Ether-a-go-go-related protein 3; hERG-3; Voltage-gated potassium channel subunit Kv11.3
产品详情
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNH7 |
| Uniprot No | Q9NS40 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1196aa |
| 活性数据 | MPVRRGHVAPQNTFLGTIIRKFEGQNKKFIIANARVQNCAIIYCNDGFCEMTGFSRPDVMQKPCTCDFLHGPETKRHDIAQIAQALLGSEERKVEVTYYHKNGSTFICNTHIIPVKNQEGVAMMFIINFEYVTDNENAATPERVNPILPIKTVNRKFFGFKFPGLRVLTYRKQSLPQEDPDVVVIDSSKHSDDSVAMKHFKSPTKESCSPSEADDTKALIQPSKCSPLVNISGPLDHSSPKRQWDRLYPDMLQSSSQLSHSRSRESLCSIRRASSVHDIEGFGVHPKNIFRDRHASEDNGRNVKGPFNHIKSSLLGSTSDSNLNKYSTINKIPQLTLNFSEVKTEKKNSSPPSSDKTIIAPKVKDRTHNVTEKVTQVLSLGADVLPEYKLQTPRINKFTILHYSPFKAVWDWLILLLVIYTAIFTPYSAAFLLNDREEQKRRECGYSCSPLNVVDLIVDIMFIIDILINFRTTYVNQNEEVVSDPAKIAIHYFKGWFLIDMVAAIPFDLLIFGSGSDETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLMLLMCIFALIAHWLACIWYAIGNVERPYLTDKIGWLDSLGQQIGKRYNDSDSSSGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHMQMLRVKEFIRFHQIPNPLRQRLEEYFQHAWTYTNGIDMNMVLKGFPECLQADICLHLNQTLLQNCKAFRGASKGCLRALAMKFKTTHAPPGDTLVHCGDVLTALYFLSRGSIEILKDDIVVAILGKNDIFGEMVHLYAKPGKSNADVRALTYCDLHKIQREDLLEVLDMYPEFSDHFLTNLELTFNLRHESAKADLLRSQSMNDSEGDNCKLRRRKLSFESEGEKENSTNDPEDSADTIRHYQSSKRHFEEKKSRSSSFISSIDDEQKPLFSGIVDSSPGIGKASGLDFEETVPTSGRMHIDKRSHSCKDITDMRSWERENAHPQPEDSSPSALQRAAWGISETESDLTYGEVEQRLDLLQEQLNRLESQMTTDIQTILQLLQKQTTVVPPAYSMVTAGSEYQRPIIQLMRTSQPEASIKTDRSFSPSSQCPEFLDLEKSKLKSKESLSSGVHLNTASEDNLTSLLKQDSDLSLELHLRQRKTYVHPIRHPSLPDSSLSTVGIVGLHRHVSDPGLPGK |
| 分子量 | 135 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
参考文献
以下是关于重组人KCNH7蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**：*Functional Expression and Electrophysiological Characterization of Recombinant Human KCNH7 Channels in HEK293 Cells*
**作者**：Smith J, Brown A, et al.
**摘要**：本研究在HEK293细胞中成功表达重组人KCNH7蛋白，通过膜片钳技术证实其作为电压门控钾离子通道的特性，并揭示其在神经元动作电位复极化中的作用。
2. **文献名称**：*Cryo-EM Structure of the Human KCNH7 Potassium Channel Reveals Unique Gating Mechanisms*
**作者**：Zhang Y, Wang L, et al.
**摘要**：利用冷冻电镜解析了重组人KCNH7蛋白的高分辨率结构，阐明了其门控调控机制及与家族成员(如KCNH2)的结构差异，为靶向药物设计提供依据。
3. **文献名称**：*KCNH7 Modulates Synaptic Plasticity and Its Dysfunction Links to Neurodevelopmental Disorders*
**作者**：Chen X, Li R, et al.
**摘要**：通过体外重组KCNH7蛋白功能实验和小鼠模型，证明KCNH7通过调节钾离子流影响突触可塑性，其突变可能导致神经发育异常。
4. **文献名称**：*High-Throughput Screening of Compounds Targeting Recombinant KCNH7 for Therapeutic Discovery*
**作者**：Kim S, Patel D, et al.
**摘要**：开发了基于重组KCNH7蛋白的药物筛选平台，鉴定出潜在调控分子，为治疗KCNH7相关神经系统疾病提供候选化合物。
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**注意**：以上为假设性示例，实际文献需通过**PubMed**、**Google Scholar**或**Web of Science**等平台以关键词“recombinant human KCNH7”“KCNH7 ion channel”等检索获取。
背景信息
The KCNH7 protein, encoded by the *KCNH7* gene in humans, belongs to the ether-à-go-go (EAG) family of voltage-gated potassium channels. These channels regulate neuronal excitability by mediating potassium ion flow during action potential repolarization. KCNH7. also known as Kv11.2. is predominantly expressed in the central nervous system, particularly in the hippocampus, cortex, and cerebellum, suggesting a role in cognitive functions and synaptic plasticity. Structurally, it features six transmembrane domains and a cyclic nucleotide-binding homology domain, facilitating voltage sensing and channel gating. Dysregulation of KCNH7 has been linked to neurological disorders, including epilepsy and schizophrenia, highlighting its physiological significance. Recombinant KCNH7 protein is engineered via heterologous expression systems (e.g., HEK293 or CHO cells) for in vitro studies. This enables researchers to explore its biophysical properties, drug interactions, and pathophysiological mechanisms. Its recombinant form is crucial for screening channel modulators, studying structure-function relationships, and developing therapeutics targeting potassium channel dysfunction. Challenges include achieving proper post-translational modifications and stable tetrameric assembly, essential for functional activity. Overall, recombinant KCNH7 serves as a vital tool for advancing neuroscience research and precision medicine.
