| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNH1 |
| Uniprot No | O95259 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-989aa |
| 活性数据 | MTMAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFMYGELTDKDTIEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPIEDDSCKGWGKFARLTRALTSSRGVLQQLAPSVQKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCVFKTTWDWIILILTFYTAILVPYNVSFKTRQNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEVISDPKLIRMNYLKTWFVIDLLSCLPYDVINAFENVDEVSAFMGDPGKIGFADQIPPPLEGRESQGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLYQLAMDIGTPYQFNGSGSGKWEGGPSKNSVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLLYATIFGNVTTIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQICPKDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEATLAQSCANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYNLRKRIVFRKISDVKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATPVSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTKASGEATLKKTDSCDSGITKSDLRLDNVGEARSPQDRSPILAEVKHSFYPIPEQTLQATVLEVRHELKEDIKALNAKMTNIEKQLSEILRILTSRRSSQSPQELFEISRPQSPESERDIFGAS |
| 分子量 | 111.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNH1蛋白的参考文献示例(基于研究领域常见主题，部分为假设性示例，供参考)：
1. **文献名称**: "Electrophysiological properties of recombinant human KCNH1 channels expressed in HEK293 cells"
**作者**: Smith, J. et al.
**摘要**: 研究通过HEK293细胞体系重组表达人KCNH1钾离子通道，分析其电压依赖性激活特性及缓慢失活动力学，揭示其在神经元动作电位复极化中的潜在作用。
2. **文献名称**: "Structural insights into the gating mechanism of KCNH1 by cryo-EM"
**作者**: Li, X. et al.
**摘要**: 利用冷冻电镜技术解析重组人KCNH1蛋白的高分辨率结构，阐明其电压感应域构象变化与通道开放的分子机制，为突变相关疾病的机制研究提供依据。
3. **文献名称**: "KCNH1 mutations impair channel function and cause developmental disorders via dominant-negative effects"
**作者**: Tanaka, R. et al.
**摘要**: 通过重组表达携带Temple-Baraitser综合征相关突变的KCNH1蛋白，证明突变导致通道功能丧失及显性负效应，从而引发神经发育异常。
4. **文献名称**: "Pharmacological profiling of recombinant KCNH1 channels for cardiac toxicity assessment"
**作者**: García, M. et al.
**摘要**: 评估多种化合物对重组KCNH1通道的抑制作用，提示该通道的异常抑制可能与药物诱导的心律失常相关，为临床安全性评价提供参考。
**注意**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词“recombinant human KCNH1”“KCNH1 expression”“KCNH1 channel structure”检索近期或高被引论文获取准确信息。
The KCNH1 protein, a member of the potassium voltage-gated channel family (Ether-à-go-go, EAG subfamily), plays a critical role in regulating cellular electrical excitability and signaling. Structurally, it features six transmembrane domains, a pore-forming loop, and a cytoplasmic cyclic nucleotide-binding domain (CNBD). Functionally, KCNH1 contributes to the delayed rectifier potassium current (IKr), influencing action potential repolarization in neurons and other excitable cells, though its expression extends to non-excitable tissues like cancer cells. Dysregulation of KCNH1 is implicated in neurological disorders (e.g., Temple-Baraitser syndrome, epilepsy) and tumor progression due to altered ion homeostasis or channelopathies. Recombinant human KCNH1 protein, typically produced via heterologous expression systems (e.g., HEK293 or insect cells), enables mechanistic studies of its gating, pharmacology, and post-translational modifications. Its recombinant form is pivotal for high-throughput drug screening to identify channel modulators for therapeutic targeting. Research also leverages this protein to explore structural determinants of voltage sensitivity and interactions with regulatory proteins, advancing insights into pathophysiological mechanisms and precision medicine strategies.
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