| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JMY |
| Uniprot No | Q8N9B5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 活性数据 | MVELLDLYQMEDEAYSSLAEATTELYQYLLQPFRDMRELAMLRRQQIKISMENDYLGPRRIESLQKEDADWQRKAHMAVLSIQDLTVKYFEITAKAQKAVYDRMRADQKKFGKASWAAAAERMEKLQYAVSKETLQMMRAKEICLEQRKHALKEEMQSLRGGTEAIARLDQLEADYYDLQLQLYEVQFEILKCEELLLTAQLESIKRLISEKRDEVVYYDTYESMEAMLEKEEMAASAYLQREELQKLQQKARQLEARRGRVSAKKSYLRNKKEICIAKHNEKIQQRTRIEDEYRTHHTVQLKREKLHDEEERKSAWVSQERQRTLDRLRTFKQRYPGQVILKSTRLRLAHARRKGAASPVLQEDHCDSLPSVLQVEEKTEEVGEGRVKRGPSQTTEPQSLVQLEDTSLTQLEATSLPLSGVTSELPPTISLPLLNNNLEPCSVTINPLPSPLPPTPPPLPVAKDSGPETLEKDLPRKEGNEKRIPKSASAPSAHLFDSSQLVSARKKLRKTAEGLQRRRVSSPMDEVLASLKRGSFHLKKVEQRTLPPFPDEDDSNNILAQIRKGVKLKKVQKDVLRESFTLLPDTDPLTRSIHEALRRIKEASPESEDEEEALPCTDWEN |
| 分子量 | 68.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人JMY蛋白的参考文献示例(内容为虚构，仅供示例参考)：
1. **文献名称**：*"Cloning and Functional Characterization of Human JMY Protein in Actin Polymerization"*
**作者**：Carnesecchi, J., et al.
**摘要**：研究报道了人JMY基因的克隆及其重组蛋白的表达，揭示了JMY通过激活Arp2/3复合体促进肌动蛋白聚合，调控细胞迁移的分子机制。
2. **文献名称**：*"JMY as a Co-activator of p53: Implications in Stress-induced Apoptosis"*
**作者**：Lin, Y., et al.
**摘要**：本文阐明了重组JMY蛋白与肿瘤抑制蛋白p53的相互作用，证明JMY通过增强p53的转录活性参与DNA损伤后的细胞凋亡调控。
3. **文献名称**：*"Recombinant JMY Facilitates DNA Repair via Interaction with Histone Modifiers"*
**作者**：Banks, L.M., et al.
**摘要**：通过体外实验验证了重组JMY蛋白在DNA损伤修复中的作用，发现其与组蛋白乙酰转移酶协同调控染色质重塑，促进修复效率。
4. **文献名称**：*"JMY Expression in Human Cancers: A Meta-Analysis of Clinical Datasets"*
**作者**：Smith, R.P., et al.
**摘要**：通过分析癌症基因组数据，发现JMY在多种肿瘤中异常表达，并探讨了重组JMY蛋白在靶向治疗中的潜在应用。
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**注意**：以上为模拟生成的示例，实际文献需通过PubMed、Web of Science等数据库检索(关键词：**JMY protein**, **recombinant JMY**, **actin nucleation**, **p53 co-activator**)。建议结合具体研究方向筛选文献。
**Background of Recombinant Human JMY Protein**
Junctional mediating and regulatory protein (JMY) is a multifunctional regulator involved in critical cellular processes such as DNA damage response, actin cytoskeleton remodeling, and transcriptional regulation. Initially identified in 1999 as a p53 coactivator, JMY interacts with p53 under stress conditions to modulate apoptosis and cell cycle arrest. Structurally, JMY contains several conserved domains, including WH2 (WASP homology 2) motifs for actin nucleation, a coiled-coil domain for protein interactions, and nuclear localization signals, enabling its dual role in the nucleus and cytoplasm.
Recombinant human JMY protein is engineered via genetic cloning, typically expressed in *E. coli* or mammalian systems, followed by purification for experimental use. Its recombinant form allows detailed study of JMY’s mechanisms in actin polymerization—via interaction with the ARP2/3 complex—and its transcriptional coactivation functions. Dysregulation of JMY is linked to cancer progression, neurodevelopmental disorders, and impaired cell migration, making it a focus in oncology and disease biomarker research. Additionally, recombinant JMY serves as a tool to explore therapeutic strategies targeting cytoskeletal dynamics or DNA damage response pathways. Studies also highlight its role in cellular stress adaptation, angiogenesis, and metastasis, underscoring its potential as a diagnostic or therapeutic target.
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