| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IPMK |
| Uniprot No | Q8NFU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 活性数据 | MATEPPSPLRVEAPGPPEMRTSPAIESTPEGTPQPAGGRLRFLNGCVPLSHQVAGHMYGKDKVGILQHPDGTVLKQLQPPPRGPRELEFYNMVYAADCFDGVLLELRKYLPKYYGIWSPPTAPNDLYLKLEDVTHKFNKPCIMDVKIGQKSYDPFASSEKIQQQVSKYPLMEEIGFLVLGMRVYHVHSDSYETENQHYGRSLTKETIKDGVSRFFHNGYCLRKDAVAASIQKIEKILQWFENQKQLNFYASSLLFVYEGSSQPTTTKLNDRTLAEKFLSKGQLSDTEVLEYNNNFHVLSSTANGKIESSVGKSLSKMYARHRKIYTKKHHSQTSLKVENLEQDNGWKSMSQEHLNGNVLSQLEKVFYHLPTGCQEIAEVEVRMIDFAHVFPSNTIDEGYVYGLKHLISVLRSILDN |
| 分子量 | 73.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于常见研究领域的模拟参考文献示例，建议通过学术数据库核实实际存在的文献：
1. **文献名称**: "Expression and Characterization of Recombinant Human Inositol Polyphosphate Multikinase"
**作者**: Smith J, Lee R, et al.
**摘要**: 该研究报道了在大肠杆菌系统中成功表达重组人IPMK蛋白，优化了纯化条件并验证了其激酶活性，证实其能催化多种肌醇磷酸底物的磷酸化。
2. **文献名称**: "Structural Insights into Human IPMK by X-ray Crystallography"
**作者**: Zhang Y, Wang H, et al.
**摘要**: 通过X射线晶体学解析了重组人IPMK的三维结构，揭示了其底物结合口袋和催化机制，为设计选择性抑制剂提供了结构基础。
3. **文献名称**: "Role of IPMK in mTOR Signaling Pathway"
**作者**: Brown K, Anderson G, et al.
**摘要**: 利用重组人IPMK蛋白进行体外实验，证实其通过生成特定的肌醇磷酸分子调控mTOR通路活性，影响细胞生长与代谢过程。
4. **文献名称**: "IPMK as a Regulator of Gene Transcription"
**作者**: Patel S, Johnson M, et al.
**摘要**: 研究发现重组人IPMK可直接与组蛋白修饰酶相互作用，在细胞核中介导基因表达调控，提示其在表观遗传学中的新功能。
**注意**: 上述条目为模拟示例，实际文献需通过PubMed或Google Scholar以关键词“recombinant human IPMK”、“hIPMK expression”等检索获取。
Inositol polyphosphate multikinase (IPMK) is a conserved enzyme central to inositol phosphate metabolism, catalyzing the phosphorylation of various inositol phosphates (IPs) to generate key signaling molecules like IP4. IP5. and IP6. These molecules regulate critical cellular processes, including chromatin remodeling, mRNA export, DNA repair, and metabolic signaling. Human IPMK also exhibits non-catalytic functions, such as acting as a transcriptional coactivator for p53 and mTOR-dependent nutrient sensing. Recombinant human IPMK (rhIPMK) is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural, enzymatic, and signaling roles. Its recombinant form enables detailed biochemical analyses, enzyme kinetics, and screening of modulators for therapeutic development. Research highlights IPMK's involvement in diseases like cancer, diabetes, and neurodegeneration, making it a potential drug target. RhIPMK's availability has advanced understanding of its dual kinase/phosphatase activity and interplay with pathways like AMPK and Akt, offering insights into metabolic disorders and aging. Studies using rhIPMK also explore its role in cellular stress responses, providing a tool to dissect IP-mediated signaling networks and their physiological impacts.
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