| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGKV1-5 |
| Uniprot No | P01602 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-235aa |
| 氨基酸序列 | MEAPAQLLFLLLLWLPDSTGEIVMTQSPATLSVSPGERATLSCRASQSISNNLAWYQQRPGQAPRLLIYGASSRVTGIPGRFSGSGSGTEFTLSISSLQSEDFAVYFCQQYNDWLLYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
| 分子量 | 52.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IGKV1-5蛋白的虚构参考文献示例(基于常见研究方向假设，非真实文献)：
---
1. **Structural Characterization of Recombinant Human IGKV1-5: Insights into Light Chain Dynamics**
*作者：Miller, A. et al.*
*摘要*：通过X射线晶体学解析了重组人IGKV1-5的结构，揭示了其与重链(IGHV)的互作界面，为抗体工程中稳定性设计提供依据。
2. **IGKV1-5 Overexpression in Autoimmune Disorders: A Biomarker Study**
*作者：Chen, L. & Gupta, S.*
*摘要*：分析了类风湿性关节炎患者血清中IGKV1-5的表达水平升高现象，探讨其作为潜在诊断标志物的可能性。
3. **Optimized Expression of Recombinant IGKV1-5 in E. coli: Purification and Functional Validation**
*作者：Yamamoto, K. et al.*
*摘要*：报道了基于大肠杆菌系统的IGKV1-5高效重组表达策略，采用亲和层析纯化并验证其抗原结合活性。
4. **IGKV1-5 in Antibody Engineering: Enhancing Affinity through Directed Evolution**
*作者：Smith, J. & Roberts, T.*
*摘要*：利用定向进化技术改造IGKV1-5可变区，优化其与肿瘤抗原的结合亲和力，推动治疗性抗体开发。
---
如需真实文献，建议在PubMed或Web of Science中以关键词“recombinant IGKV1-5”“human kappa light chain”等检索，或结合特定研究领域(如疾病关联、结构生物学)筛选。
Recombinant human IGKV1-5 protein is a engineered form of the immunoglobulin kappa variable region 1-5 (IGKV1-5), a component of antibody light chains. In humans, immunoglobulins (antibodies) consist of heavy and light chains, with the light chains subdivided into kappa (κ) and lambda (λ) types. The IGKV1 gene cluster on chromosome 2 encodes variable regions of kappa light chains, which determine antigen-binding specificity through combinatorial diversity. The IGKV1-5 segment specifically represents one of the variable gene segments within the IGKV1 subgroup.
Recombinant IGKV1-5 is produced using biotechnological methods, such as expression in bacterial or mammalian systems, followed by purification. This protein retains the variable domain's structural features, including complementarity-determining regions (CDRs) critical for antigen recognition. It serves as a tool in antibody engineering, immunology research, and therapeutic development, particularly in studying B-cell receptor diversity, antibody maturation, and autoimmune disorders linked to kappa chain anomalies. Additionally, recombinant IGKV1-5 may aid in designing monoclonal antibodies or bispecific molecules, as well as in biomarker discovery for diseases involving aberrant B-cell activity. Its availability facilitates standardized studies on antibody-antigen interactions without reliance on native antibody isolates.
×
