| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGHM |
| Uniprot No | P01871 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489aa |
| 氨基酸序列 | MNYYWGQRTLVTVSSGSASAPTLFPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK |
| 分子量 | 80.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IGHM蛋白研究的3篇示例参考文献(内容为虚构，仅供示例)：
1. **文献名称**：*Expression and Purification of Recombinant Human IGHM in Mammalian Cells*
**作者**：Zhang L. et al.
**摘要**：研究利用HEK293细胞系统高效表达重组人IGHM蛋白，优化纯化流程并通过质谱验证结构完整性，为免疫学研究提供标准化的蛋白制备方案。
2. **文献名称**：*Structural Insights into Recombinant IGHM and Its Antigen-Binding Capacity*
**作者**：Johnson R.B. et al.
**摘要**：通过X射线晶体学解析重组人IGHM的Fc区域三维结构，揭示其与病原体抗原结合的分子机制，为抗体工程提供结构基础。
3. **文献名称**：*Functional Analysis of Recombinant IGHM in B Cell Signaling*
**作者**：Kuroda S. et al.
**摘要**：探讨重组人IGHM在体外B细胞受体信号激活中的作用，证实其可模拟天然IgM增强免疫应答的能力，为免疫疗法开发提供依据。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“recombinant human IGHM”获取最新研究。
Recombinant human IGHM protein refers to the genetically engineered form of the immunoglobulin heavy chain mu (IgHM), a critical component of IgM antibodies. IgM is the first antibody isotype produced during B cell development and immune responses, existing as a membrane-bound B cell receptor (BCR) on naïve B cells or a secreted pentamer with potent pathogen-neutralizing capacity. The IGHM gene encodes the heavy chain constant region (CH) of IgM, which includes four constant domains (Cμ1-Cμ4) that mediate effector functions like complement activation.
Recombinant IGHM is typically produced in mammalian expression systems to ensure proper glycosylation and structural fidelity. It serves as a vital tool for studying B cell maturation, as surface IgM expression is a hallmark of functional BCR signaling and a checkpoint during B cell development. Researchers use it to investigate immunodeficiencies (e.g., agammaglobulinemia linked to IGHM mutations), autoimmune disorders, and B cell receptor signaling mechanisms. In therapeutic contexts, recombinant IgM-based molecules are being explored for enhanced pathogen binding or cancer targeting due to their high avidity. Additionally, it aids in standardizing diagnostic assays for lymphoma subtypes expressing IgM surface markers.
The production and characterization of recombinant IGHM underscore its dual role in advancing both basic immunology research and translational applications in disease diagnosis/therapy.
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