| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIC2 |
| Uniprot No | Q96JB3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615aa |
| 氨基酸序列 | MVSGPLALRW CAWAGRGDMG PDMELPSHSK QLLLQLNQQR TKGFLCDVII MVENSIFRAH KNVLAASSIY FKSLVLHDNL INLDTDMVSS TVFQQILDFI YTGKLLPSDQ PAEPNFSTLL TAASYLQLPE LAALCRRKLK RAGKPFGSGR AGSTGMGRPP RSQRLSTASV IQARYQGLVD GRKGAHAPQE LPQAKGSDDE LFLGGSNQDS VQGLGRAVCP AGGEAGLGGC SSSTNGSSGG CEQELGLDLS KKSPPLPPAT PGPHLTPDDA AQLSDSQHGS PPAASAPPVA NSASYSELGG TPDEPMDLEG AEDNHLSLLE APGGQPRKSL RHSTRKKEWG KKEPVAGSPF ERREAGPKGP CPGEEGEGVG DRVPNGILAS GAGPSGPYGE PPYPCKEEEE NGKDASEDSA QSGSEGGSGH ASAHYMYRQE GYETVSYGDN LYVCIPCAKG FPSSEQLNAH VETHTEEELF IKEEGAYETG SGGAEEEAED LSAPSAAYTA EPRPFKCSVC EKTYKDPATL RQHEKTHWLT RPFPCNICGK MFTQRGTMTR HMRSHLGLKP FACDECGMRF TRQYRLTEHM RVHSGEKPYE CQLCGGKFTQ QRNLISHLRM HTSPS |
| 分子量 | 66.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于模拟生成的3篇关于重组人HIC2蛋白的参考文献示例(文献可能存在虚构，仅供参考)：
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1. **标题**：*Cloning and Functional Analysis of Recombinant Human HIC2 Protein in Cardiac Development*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了人源HIC2基因重组表达载体，通过大肠杆菌系统高效表达并纯化HIC2蛋白。功能实验表明，重组HIC2通过调控心肌细胞分化相关基因(如NKX2-5)的表达参与心脏发育过程。
2. **标题**：*Structural Characterization of HIC2 Protein and Its Interaction with DNA*
**作者**：Zhang Y, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度重组HIC2蛋白，通过X射线晶体学解析其三维结构，揭示了其锌指结构域与靶DNA结合的关键位点，为研究HIC2在表观遗传调控中的作用提供结构基础。
3. **标题**：*Recombinant HIC2 as a Potential Tumor Suppressor in Breast Cancer*
**作者**：Patel R, et al.
**摘要**：通过哺乳动物细胞表达系统制备重组HIC2蛋白，发现其能够抑制乳腺癌细胞增殖并诱导凋亡。机制研究表明HIC2通过结合p53启动子区域增强其转录活性，提示其抑癌潜力。
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**注**：以上文献为模拟生成，实际研究请查询权威数据库(如PubMed、Google Scholar)并核实信息。若需真实文献，建议以“HIC2 protein recombinant”或“HIC2 overexpression”为关键词检索。
The human HIC2 (hypermethylated in cancer 2) protein, encoded by the *HIC2* gene, is a zinc-finger transcriptional regulator implicated in chromatin remodeling, gene expression regulation, and cellular differentiation. Initially identified as a candidate tumor suppressor, HIC2 resides on chromosome 22q11.2. a region frequently altered in cancers and congenital disorders like DiGeorge syndrome. Structurally, it contains five C2H2-type zinc-finger motifs that mediate DNA binding and protein-protein interactions, particularly with transcriptional repressors such as BS69 and components of the Polycomb repressive complex. HIC2 plays critical roles in embryonic development, notably in heart and neural tube formation, and regulates genes involved in cell cycle control, apoptosis, and stress responses. Dysregulation of HIC2. through hypermethylation or deletions, has been linked to tumorigenesis, cardiovascular anomalies, and neurodevelopmental disorders. Recombinant human HIC2 protein, typically produced in *E. coli* or mammalian expression systems with tags like His or GST, enables functional studies, including DNA-binding assays, interactome mapping, and mechanistic exploration of its role in epigenetic silencing or activation. Its study provides insights into disease mechanisms and potential therapeutic targets.
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