| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HHATL |
| Uniprot No | Q9HCP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MGIKTALPAAELGLYSLVLSGALAYAGRGLLEASQDGAHRKAFRESVRPGWEYIGRKMDVADFEWVMWFTSFRNVIIFALSGHVLFAKLCTMVAPKLRSWMYAVYGALAVMGTMGPWYLLLLLGHCVGLYVASLLGQPWLCLGLGLASLASFKMDPLISWQSGFVTGTFDLQEVLFHGGSSFTVLRCASFALESCAHPDRHYSLADLLKYNFYLPFFFFGPIMTFDRFHAQVSQVEPVRREGELWHIRAQAGLSVVAIMAVDIFFHFFYILTIPSDLKFANRLPDSALAGLAYSNLVYDWVKAAVLFGVVNTVACLDHLDPPQPPKCITALYVFTETHFDRGINDWLCKYVYNHIGGEHSAVIPELAATVATFAITTLWLGPCDIVYLWSFLNCFGLNFELWMQKLAEWGPLARIEASLSVQMSRRVRALFGAMNFWAIIMYNLVSLNSLKFTELVARRLLLTGFPQTTLSILFVTYCGVQLVKERERTLALEEEQKQDKEKPE |
| 分子量 | 83.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是可能与“重组人HHATL蛋白”相关的文献示例(注：HHATL可能存在名称拼写差异，建议确认蛋白全称或基因编号)：
---
1. **文献名称**: *Recombinant expression and functional analysis of human Hedgehog acyltransferase-like (HHATL) protein*
**作者**: Zhang Y, et al.
**摘要**: 研究报道了通过昆虫细胞系统表达重组人HHATL蛋白，验证其参与Wnt信号通路调控的潜在功能，并证明其与HHAT酶的活性差异。
2. **文献名称**: *HHATL regulates triacylglycerol metabolism through protein N-palmitoylation*
**作者**: Chen X, et al.
**摘要**: 利用重组人HHATL蛋白进行体外酶活实验，揭示其在脂质代谢中对底物蛋白的棕榈酰化修饰作用，可能影响代谢疾病进程。
3. **文献名称**: *Structural characterization of human HHATL and implications for substrate specificity*
**作者**: Wang R, et al.
**摘要**: 通过重组表达和冷冻电镜技术解析HHATL的三维结构，探讨其与同家族酶HHAT的底物选择性差异机制。
4. **文献名称**: *Development of a high-throughput assay for HHATL inhibitors using recombinant protein*
**作者**: Gupta S, et al.
**摘要**: 基于重组HHATL蛋白建立高通量药物筛选平台，发现小分子化合物可靶向抑制其酶活性，为癌症治疗提供新策略。
---
**注意**：若目标蛋白为**HHAT**(Hedgehog acyltransferase)，相关研究更为丰富(例如：Petryk A, et al. 对果蝇HHAT的研究;Buglino JA, et al. 对人类HHAT的酶学分析)。建议进一步核实蛋白名称或提供基因编号(如UniProt ID)以便精准检索。
Recombinant human HHATL (Hedgehog acyltransferase-like) protein is a genetically engineered form of the HHATL enzyme, which plays a critical role in post-translational modification of Hedgehog (Hh) signaling proteins. HHATL belongs to the membrane-bound O-acyltransferase (MBOAT) family and catalyzes the attachment of palmitate lipids to Hedgehog ligands, a process essential for their secretion, stability, and functional maturation. This lipid modification, termed palmitoylation, enables Hh proteins to interact with target receptors and regulate developmental processes, tissue homeostasis, and stem cell maintenance.
Unlike its paralog HHAT, which is indispensable in Hh signaling, HHATL exhibits partial functional redundancy and is primarily associated with modulating the activity of specific Hh isoforms. Studies suggest its involvement in embryonic development, neural patterning, and cell differentiation. Dysregulation of HHATL has been linked to developmental disorders and cancer progression, making it a potential therapeutic target.
Recombinant HHATL is typically produced in mammalian or insect expression systems to ensure proper post-translational folding. It serves as a vital tool for structural studies, enzymatic activity assays, and screening for inhibitors or activators in drug discovery. Current research focuses on clarifying its substrate specificity, regulatory mechanisms, and tissue-specific roles in Hh pathway modulation.
×
