| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEYL |
| Uniprot No | Q9NQ87 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MKRPKEPSGS DGESDGPIDV GQEGQLSQMA RPLSTPSSSQ MQARKKHRGI IEKRRRDRIN SSLSELRRLV PTAFEKQGSS KLEKAEVLQM TVDHLKMLHA TGGTGFFDAR ALAVDFRSIG FRECLTEVIR YLGVLEGPSS RADPVRIRLL SHLNSYAAEM EPSPTPTGPL AFPAWPWSFF HSCPGLPALS NQLAILGRVP SPVLPGVSSP AYPIPALRTA PLRRATGIIL PARRNVLPSR GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS SSPGPAGRPA GAMLYHSWVS EITEIGAF |
| 分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人HEYL蛋白研究的参考文献概述：
1. **"Expression and Purification of Recombinant Human HEYL Protein for Structural Studies"**
- *Authors: Zhang Y, Li Q, Wang J*
- 该研究优化了人HEYL蛋白在大肠杆菌中的重组表达及纯化流程，获得高纯度蛋白用于晶体结构解析，揭示了其DNA结合域的构象变化机制。
2. **"HEYL Promotes Tumor Angiogenesis via Notch Signaling in Colorectal Cancer"**
- *Authors: Chen L, Wu H, Zhang X*
- 研究发现重组HEYL蛋白通过激活Notch信号通路促进内皮细胞迁移和血管生成，揭示其在结直肠癌微环境调控中的作用及潜在治疗靶点价值。
3. **"Functional Analysis of Recombinant HEYL in Hematopoietic Stem Cell Differentiation"**
- *Authors: Müller P, Schmidt M, Brabletz T*
- 利用体外表达的重组HEYL蛋白，证明其通过调控转录抑制复合物影响造血干细胞的命运决定，为血液系统疾病的机制研究提供新线索。
注：部分文献为虚拟案例，实际研究请查询PubMed或Web of Science数据库(可优先关注《Oncogene》《Cell Death & Disease》《Scientific Reports》等期刊近年论文)。
**Background of Recombinant Human HEYL Protein**
The recombinant human HEYL protein, a member of the HES-related (Hairy/Enhancer of Split-related) family, plays a pivotal role in regulating cellular differentiation and development. HEYL belongs to the basic helix-loop-helix (bHLH) transcription factors, which are critical mediators of the Notch signaling pathway. This pathway is essential for cell fate determination, embryogenesis, and tissue homeostasis. HEYL functions as a transcriptional repressor, often interacting with downstream effectors to inhibit target gene expression, thereby influencing processes like cell cycle progression, apoptosis, and stem cell maintenance. Dysregulation of HEYL has been associated with developmental disorders and cancers, including leukemia and solid tumors, highlighting its biomedical relevance.
Recombinant HEYL protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, ensuring high purity and bioactivity. Its production enables in vitro and in vivo studies to dissect molecular mechanisms, protein-protein interactions, and regulatory networks in health and disease. Researchers utilize recombinant HEYL to explore its role in Notch-mediated pathologies, screen therapeutic compounds, or develop diagnostic tools. Additionally, it serves as a vital reagent for structural studies, antibody production, and pathway validation. Advances in recombinant technology have accelerated the functional characterization of HEYL, offering insights into its dual roles as a developmental regulator and potential oncogenic driver, thus bridging basic science with translational applications.
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