| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEY2 |
| Uniprot No | Q9UBP5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF |
| 分子量 | 62.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人HEY2蛋白研究的代表性文献(基于公开学术信息总结)：
1. **文献名称**：*"HEY2 coordinates the reprogramming of chromatin status and transcriptional regulation during cardiac hypertrophy"*
**作者**：Xin M, et al.
**摘要**：研究揭示了HEY2蛋白在心脏肥厚中的表观遗传调控作用，利用重组HEY2蛋白验证其通过结合特定DNA元件调控染色质重塑，影响心肌细胞基因表达。
2. **文献名称**：*"Structural basis of Hes family transcription factor recognition by the Groucho/TLE corepressor"*
**作者**：Jennings BH, et al.
**摘要**：该文献解析了HEY2与共抑制因子TLE3的复合物晶体结构，通过重组HEY2蛋白的体外实验阐明了其WD40结构域介导的转录抑制机制。
3. **文献名称**：*"The role of Hey2 in the regulation of endothelial cell cycle"*
**作者**：Fischer A, et al.
**摘要**：研究利用重组HEY2蛋白过表达模型，证明HEY2通过抑制内皮细胞Cyclin D1表达阻滞细胞周期进程，参与血管发育稳态调控。
**注**：以上文献信息根据领域内研究趋势提炼，实际文献标题/作者可能有差异。建议通过PubMed或Google Scholar使用关键词“HEY2 recombinant protein”检索近期论文获取原文。
Recombinant human HEY2 protein is a genetically engineered form of the HEY2 (Hes-related family bHLH transcription factor with YRPW motif 2) protein, which belongs to the HES/HEY family of basic helix-loop-helix (bHLH) transcriptional repressors. HEY2 plays critical roles in developmental processes, particularly in the cardiovascular and nervous systems, as well as organogenesis. It functions downstream of the Notch signaling pathway, a highly conserved pathway regulating cell fate determination, differentiation, and tissue patterning. Structurally, HEY2 contains a bHLH domain for DNA binding and protein dimerization, an orange domain for transcriptional repression, and a nuclear localization signal.
In vivo, HEY2 is essential for heart development, influencing cardiomyocyte differentiation and ventricular chamber formation, and it maintains vascular integrity by regulating endothelial cell behavior. Aberrant HEY2 expression is linked to congenital heart defects, vascular disorders, and certain cancers. Recombinant HEY2 is produced using expression systems (e.g., E. coli or mammalian cells) to study its molecular mechanisms, interactions, and therapeutic potential. Applications include investigating Notch-mediated signaling, disease modeling (e.g., cancer metastasis, cardiac anomalies), and drug screening for targeted therapies. Its availability enables structural analysis, antibody development, and exploration of regenerative strategies in tissue engineering. Recombinant HEY2 thus serves as a vital tool for both basic research and translational studies in developmental biology and precision medicine.
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