| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GANC |
| Uniprot No | Q8TET4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-914aa |
| 氨基酸序列 | MEAAVKEEISVEDEAVDKNIFRDCNKIAFYRRQKQWLSKKSTYRALLDSVTTDEDSTRFQIINEASKVPLLAEIYGIEGNIFRLKINEETPLKPRFEVPDVLTSKPSTVRLISCSGDTGSLILADGKGDLKCHITANPFKVDLVSEEEVVISINSLGQLYFEHLQILHKQRAAKENEEETSVDTSQENQEDLGLWEEKFGKFVDIKANGPSSIGLDFSLHGFEHLYGIPQHAESHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLAHKLGRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVFLLTGPTPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPYDAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYVKAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFLWNDMNEPSVFRGPEQTMQKNAIHHGNWEHRELHNIYGFYHQMATAEGLIKRSKGKERPFVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNPETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYSLFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALLVHPVTEPKATTVDVFLPGSNEVWYDYKTFAHWEGGCTVKIPVALDTIPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRVALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKFSFCSSVLINSFADQRGHYPSKCVVEKILVLGFRKEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKLSLNIATDWEVRII |
| 分子量 | 127.05 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GANC蛋白的3篇示例参考文献(注：实际文献可能需要通过数据库检索确认，以下为模拟示例)：
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1. **文献名称**：Recombinant Human Neutral Alpha-Glucosidase C (GANC) Production in CHO Cells and Functional Characterization
**作者**：Smith A, et al.
**摘要**：本研究描述了在CHO细胞中高效表达重组人GANC蛋白的流程，并通过质谱和酶活性分析验证其结构与功能，证实其在体外可降解糖原底物，为酶替代疗法提供基础。
2. **文献名称**：Crystal Structure and Substrate Specificity of Recombinant Human GANC Protein
**作者**：Zhang L, et al.
**摘要**：解析了重组人GANC蛋白的晶体结构，阐明其底物结合域的关键氨基酸残基，发现其对多糖类底物的特异性水解活性，提示其在代谢疾病治疗中的潜在应用。
3. **文献名称**：Development of a GANC Enzyme Assay for Diagnosis of Glycogen Storage Disease Variants
**作者**：Johnson R, et al.
**摘要**：开发了一种基于重组人GANC蛋白的体外诊断方法，用于检测因GANC缺乏导致的罕见糖原贮积症，临床样本验证显示高敏感性和特异性。
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**建议**：实际文献检索推荐使用PubMed、Google Scholar等平台，以“recombinant human GANC protein”“neutral alpha-glucosidase C”为关键词，或结合相关疾病(如糖原贮积症)进行精准搜索。
Recombinant human GANC (glucosidase alpha neutral C) protein is a genetically engineered form of the endogenous enzyme produced through biotechnology platforms. GANC belongs to the glycosyl hydrolase family and is involved in carbohydrate metabolism, particularly in hydrolyzing terminal alpha-1.4-glucose linkages in glycogen and oligosaccharides. Unlike its lysosomal counterpart, acid alpha-glucosidase (GAA), GANC operates in a neutral pH environment, suggesting distinct cellular roles, potentially in cytosolic or ER-associated glycogen breakdown.
Interest in recombinant GANC stems from its implications in metabolic disorders. Mutations in GANC are linked to rare glycogen storage diseases (GSDs) and may contribute to type 2 diabetes or neurodegenerative conditions by affecting glucose homeostasis. Recombinant expression systems (e.g., E. coli, mammalian cells) enable large-scale production of purified GANC for functional studies, structural analysis, and therapeutic exploration. Its recombinant form is critical for studying enzyme kinetics, substrate specificity, and interactions with other metabolic regulators.
Additionally, recombinant GANC holds therapeutic potential as an enzyme replacement therapy (ERT) for GSDs or as a modulator of glycogen dynamics in metabolic syndromes. Research also focuses on its role in cellular stress responses, autophagy, and cancer metabolism. By providing a consistent, high-purity enzyme source, recombinant GANC accelerates translational studies, bridging molecular insights to clinical applications.
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