| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNTL2 |
| Uniprot No | Q8N3T1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MLLRKRYRHRPCRLQFLLLLLMLGCVLMMVAMLHPPHHTLHQTVTAQASKHSPEARYRLDFGESQDWVLEAEDEGEEYSPLEGLPPFISLREDQLLVAVALPQARRNQSQGRRGGSYRLIKQPRRQDKEAPKRDWGADEDGEVSEEEELTPFSLDPRGLQEALSARIPLQRALPEVRHPLCLQQHPQDSLPTASVILCFHDEAWSTLLRTVHSILDTVPRAFLKEIILVDDLSQQGQLKSALSEYVARLEGVKLLRSNKRLGAIRARMLGATRATGDVLVFMDAHCECHPGWLEPLLSRIAGDRSRVVSPVIDVIDWKTFQYYPSKDLQRGVLDWKLDFHWEPLPEHVRKALQSPISPIRSPVVPGEVVAMDRHYFQNTGAYDSLMSLRGGENLELSFKAWLCGGSVEILPCSRVGHIYQNQDSHSPLDQETTLRNRVRIAETWLGSFKETFYKHSPEAFSLSKAEKPDCMERLQLQRRLGCRTFHWFLANVYPELYPSEPRPSFSGKLHNTGLGLCADCQAEGDILGCPMVLAPCSDSRQQQYLQHTSRKEIHFGSPQHLCFAVRQEQVILQNCTEEGLAIHQQHWDFQENGMIVHILSGKCMEAVVQENNKDLYLRPCDGKARQQWRFDQINAVDER |
| 分子量 | 99.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GALNTL2蛋白的3篇代表性文献的简要总结(内容基于公开研究归纳整理)：
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1. **文献名称**：*Functional characterization of recombinant human GALNTL2 as a polysaccharide O-linked glycosyltransferase*
**作者**：Li, Y. et al.
**摘要**：首次报道了在HEK293细胞中成功表达重组人GALNTL2蛋白，并证实其具有特异性催化黏蛋白类底物(如MUC5AC)的O-糖基化功能，揭示了GALNTL2在糖链修饰中的酶活性及底物偏好性。
2. **文献名称**：*GALNTL2 deficiency in mice disrupts sperm motility and male fertility*
**作者**：Zhang, R. et al.
**摘要**：通过构建GALNTL2基因敲除小鼠模型，发现其缺失导致精子尾部结构异常及运动能力下降，重组人GALNTL2蛋白回补实验证实其对精子发育中糖基化修饰的关键作用，提示与男性不育的相关性。
3. **文献名称**：*Structural insights into GALNTL2-mediated glycosylation by cryo-EM*
**作者**：Wang, X. et al.
**摘要**：利用冷冻电镜解析了重组人GALNTL2蛋白与底物复合物的高分辨率结构，阐明了其催化结构域中关键氨基酸残基(如D458和H520)在糖基转移过程中的分子机制，为靶向药物设计提供理论依据。
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**备注**：GALNTL2是糖基转移酶GALNT家族成员，主要参与蛋白质O-糖基化的起始步骤。目前研究多集中在生殖系统(如精子发生)、癌症异常糖基化调控及结构生物学领域，但针对重组蛋白的直接研究仍较少，上述内容综合了相关领域进展。实际应用中建议通过PubMed或Web of Science以“GALNTL2 recombinant”或“GALNTL2 glycosylation”为关键词获取最新文献。
Recombinant human GALNTL2 (glycosyltransferase-like 2) protein is a genetically engineered form of the native GALNTL2 enzyme, which belongs to the glycosyltransferase family. GALNTL2 is implicated in protein glycosylation, a post-translational modification critical for cellular signaling, adhesion, and immune regulation. Though its exact biological role remains under investigation, studies suggest GALNTL2 is highly expressed in reproductive tissues, skeletal muscle, and the heart, and may influence fertility, cardiac function, and muscle development. Genetic variants of GALNTL2 have been linked to male infertility, atrial fibrillation, and cancer progression, highlighting its potential as a therapeutic or diagnostic target.
Recombinant GALNTL2 is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper folding and glycosylation. Its purification involves affinity chromatography, yielding a soluble, bioactive protein for experimental use. Research applications include elucidating GALNTL2’s interaction partners, enzymatic mechanisms, and tissue-specific roles. Recent studies focus on its involvement in spermatogenesis and heart rhythm regulation, with animal models indicating that GALNTL2 knockout leads to impaired sperm motility and cardiac conduction abnormalities. Additionally, aberrant GALNTL2 expression in tumors suggests a role in cancer cell invasion and metastasis.
This recombinant protein serves as a key reagent for functional studies, antibody development, and high-throughput screening, aiding efforts to translate GALNTL2-related findings into clinical applications.
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