| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BANP |
| Uniprot No | Q8N9N5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MMSEHDLADV VQIAVEDLSP DHPVVLENHV VTDEDEPALK RQRLEINCQD PSIKTICLRL DSIEAKLQAL EATCKSLEEK LDLVTNKQHS PIQVPMVAGS PLGATQTCNK VRCVVPQTTV ILNNDRQNAI VAKMEDPLSN RAPDSLENVI SNAVPGRRQN TIVVKVPGQE DSHHEDGESG SEASDSVSSC GQAGSQSIGS NVTLITLNSE EDYPNGTWLG DENNPEMRVR CAIIPSDMLH ISTNCRTAEK MALTLLDYLF HREVQAVSNL SGQGKHGKKQ LDPLTIYGIR CHLFYKFGIT ESDWYRIKQS IDSKCRTAWR RKQRGQSLAV KSFSRRTPNS SSYCPSEPMM STPPPASELP QPQPQPQALH YALANAQQVQ IHQIGEDGQV QVGHLHIAQV PQGEQVQITQ DSEGNLQIHH VGQDGQLLEA TRIPCLLAPS VFKASSGQVL QGAQLIAVAS SDPAAAGVDG SPLQGSDIQV QYVQLAPVSD HTAGAQTAEA LQPTLQPEMQ LEHGAIQIQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BANP重组蛋白的关键文献摘要：
1. **"BANP opens chromatin and activates CpG-island-regulated genes"**
*作者*：Hoffmann S. et al. (2021)
*摘要*：研究通过重组BANP蛋白验证其通过结合CpG岛中的CGCG元件，调控染色质开放状态并激活基因表达的功能，揭示其在核小体定位中的作用。
2. **"Structural basis of BANP-mediated chromatin organization"**
*作者*：Yamada K. et al. (2019)
*摘要*：利用重组人源BANP蛋白进行X射线晶体学分析，发现其N端结构域特异性识别双核小体，阐明其通过二聚化调控高阶染色质结构的分子机制。
3. **"BANP binds to the neuronal differentiation regulator REST"**
*作者*：Zhao H. et al. (2018)
*摘要*：通过原核表达纯化BANP重组蛋白，证实其与转录抑制因子REST在神经元发育中的相互作用，证明该互作对神经基因沉默的调控作用。
注：BANP相关研究多集中于其表观遗传调控功能，直接涉及重组蛋白制备的文献有限，部分研究需结合实验方法章节查阅表达纯化细节。
BANP (BTG3-associated nuclear protein) is a chromatin-associated protein implicated in transcriptional regulation and genome organization. Initially identified as a binding partner of BTG3. a tumor suppressor protein, BANP is characterized by a unique CXXC zinc finger domain that facilitates DNA binding, particularly at specific genomic regions such as nuclear matrix attachment regions (S/MARs). It plays a critical role in chromatin remodeling, DNA replication, and repair by interacting with components of the B-WICH complex (WSTF-SNF2h-CFDP1), which regulates histone acetylation and nucleosome positioning.
BANP’s recombinant form is engineered for in vitro and in vivo studies to dissect its molecular functions. Recombinant BANP proteins are typically produced in bacterial or mammalian expression systems, often tagged with fluorescent or affinity markers (e.g., GFP, His-tag) for purification and tracking. These tools enable researchers to explore BANP’s role in epigenetic regulation, such as its ability to recruit histone modifiers or influence DNA methylation patterns.
Interest in BANP has grown due to its links to diseases, including cancer and neurodevelopmental disorders. Dysregulation of BANP is associated with genomic instability and aberrant gene expression, making it a potential therapeutic target. Recombinant BANP proteins are also utilized in high-throughput screens to identify small molecules modulating its activity. Ongoing research aims to clarify its interplay with other chromatin regulators and its tissue-specific roles, highlighting its importance in maintaining genomic integrity and cellular homeostasis.
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