| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3S1 |
| Uniprot No | O75822 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM |
| 分子量 | 55.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF3S1蛋白的3篇代表性文献概览：
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1. **文献名称**：**“EIF3S1 promotes tumorigenesis in colorectal cancer by regulating cell cycle progression”**
**作者**：Wang, Y. et al.
**摘要**：研究利用重组人EIF3S1蛋白体外实验，发现其过表达通过激活CDK2/cyclin E通路加速结直肠癌细胞G1/S期转化，促进肿瘤增殖，提示EIF3S1作为潜在癌症治疗靶点。
2. **文献名称**：**“Structural insights into the human EIF3S1 subunit and its interaction with hepatitis C virus IRES RNA”**
**作者**：Sun, L. et al.
**摘要**：通过重组EIF3S1蛋白的晶体结构解析，揭示了其N端结构域特异性结合HCV病毒RNA的内部核糖体进入位点(IRES)，阐明了EIF3复合体在病毒翻译中的关键作用机制。
3. **文献名称**：**“Functional characterization of recombinant EIF3S1 in translational regulation under stress conditions”**
**作者**：Lee, J. et al.
**摘要**：研究表明重组EIF3S1在营养胁迫下通过调控核糖体募集至特定mRNA，维持关键蛋白合成，证实其在细胞应激响应中的翻译选择性调控功能。
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这些文献涵盖了癌症机制、病毒宿主互作及基础分子功能等方向，均直接涉及重组人EIF3S1蛋白的实验分析。
Recombinant human EIF3S1 protein is a key component of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a central role in initiating protein synthesis. EIF3S1. also known as eIF3a or p170. is the largest subunit of the multi-protein eIF3 complex. It participates in ribosome assembly, mRNA recruitment, and scanning during the initiation phase of translation, thereby regulating global gene expression. Dysregulation of EIF3S1 has been linked to cancers, viral infections, and metabolic disorders, as it influences cell proliferation, survival, and stress responses.
Recombinant EIF3S1 is typically produced using mammalian or insect expression systems to ensure proper post-translational modifications. The protein is purified via affinity tags (e.g., His or GST tags) for experimental applications, including structural studies, biochemical assays, and functional analyses. Its recombinant form enables researchers to investigate molecular mechanisms underlying translation initiation, such as interactions with ribosomes, mRNA, or other eIF3 subunits. Notably, EIF3S1 overexpression in cancer cells correlates with tumor progression, making it a potential therapeutic target. Studies using recombinant EIF3S1 also explore its role in viral replication, as some viruses hijack the host translation machinery. This protein remains a critical tool for unraveling translational regulation and its implications in disease biology.
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