| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2S2 |
| Uniprot No | P20042 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN |
| 分子量 | 64.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF2S2蛋白的3篇代表性文献的简要总结(文献为假设示例，实际文献需查询数据库)：
1. **文献名称**：*Expression and structural analysis of human EIF2S2 in translational regulation*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统重组表达人EIF2S2蛋白，解析其晶体结构，证实其通过GTP结合域参与真核翻译起始复合体的组装。
2. **文献名称**：*Functional characterization of EIF2S2 mutations in cellular stress response*
**作者**：Chen L, et al.
**摘要**：探讨重组EIF2S2及其突变体对细胞应激下eIF2复合体活性的影响，发现特定突变导致蛋白质翻译错误调控并与疾病相关。
3. **文献名称**：*Role of recombinant EIF2S2 in neurodegenerative disease models*
**作者**：Wang Y, et al.
**摘要**：利用重组EIF2S2蛋白干预阿尔茨海默病细胞模型，证明其过表达可缓解内质网应激诱导的神经元凋亡。
如需真实文献，建议检索PubMed或Web of Science，关键词“recombinant human EIF2S2”或“eIF2β subunit function”。
Recombinant human EIF2S2 protein, also known as eukaryotic translation initiation factor 2 subunit beta, is a critical component of the eIF2 complex, which regulates the initiation of protein synthesis in eukaryotic cells. The eIF2 complex binds GTP and Met-tRNA to the 40S ribosomal subunit, a fundamental step in translation. EIF2S2 serves as the β-subunit of the heterotrimeric eIF2 (composed of α, β, and γ subunits) and plays a role in modulating the GTP/GDP exchange activity of eIF2. a process tightly regulated by kinases like PKR and HRI under stress conditions. Dysregulation of EIF2S2 or its phosphorylation status is implicated in cellular stress responses, oncogenesis, and neurological disorders.
The recombinant form is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure proper post-translational modifications for functional studies. It is widely used to investigate translation mechanisms, eIF2-related signaling pathways (e.g., integrated stress response), and interactions with regulatory proteins or small molecules. Researchers also utilize recombinant EIF2S2 to explore mutations linked to developmental defects or diseases, such as MEHMO syndrome, a rare X-linked disorder characterized by intellectual disability and epilepsy. Its availability aids in drug discovery targeting translation dysregulation in cancer or neurodegenerative diseases like Alzheimer's.
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