| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNJ |
| Uniprot No | Q5T5M9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MELEGQWWRGQLAADIHQALRYKELKLPSYKGQSPQLSLRRYFADLIAIVSNRFTLCPSARHLAVYLLDLFMDRYDISIQQLHLVALSCLLLASKFEEKEDSVPKLEQLNSLGCMTNMNLVLTKQNLLHMELLLLETFQWNLCLPTAAHFIEYYLSEAVHETDLHDGWPMICLEKTKLYMAKYADYFLEVSLQVAAACVASSRIILRLSPTWPTRLHRLTAYSWDFLVQCIERLLIAHDNDVKEANKQRGQAGPQSAQLSVFQTASQPSRPVHFQQPQYLHQTHQTSLQYRHPTSEQPSCQQIVSTTHTSSYTLQTCPAGFQTSVQGLGHMQTGVGMSLAIPVEVKPCLSVSYNRSYQINEHYPCITPCFER |
| 分子量 | 66.66 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4条关于重组人周期素J(CCNJ)蛋白的参考文献示例(文献内容基于模拟场景整理)：
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1. **标题**：*"Functional Characterization of Recombinant Human Cyclin J in Cell Cycle Regulation"*
**作者**：Zhang Y. et al.
**摘要**：研究利用哺乳动物细胞系统表达重组CCNJ蛋白，证实其与CDK10相互作用，调控G2/M期进程，并参与RNA聚合酶II的转录延伸调控。
2. **标题**：*"Efficient Expression and Purification of Recombinant CCNJ in E. coli for Structural Analysis"*
**作者**：Li X. et al.
**摘要**：优化原核表达体系(大肠杆菌BL21)，纯化重组CCNJ蛋白并通过质谱和圆二色谱验证其结构，为后续功能研究提供基础。
3. **标题**：*"Cyclin J-CDK10 Complex Modulates Transcriptional Pausing in Stem Cells"*
**作者**：Müller S. et al.
**摘要**：通过体外重组实验，发现CCNJ-CDK10复合物磷酸化RNA聚合酶II C端结构域，影响胚胎干细胞分化过程中的基因表达调控。
4. **标题**：*"Overexpression of Recombinant CCNJ Correlates with Poor Prognosis in Breast Cancer"*
**作者**：Wang L. et al.
**摘要**：利用重组CCNJ蛋白进行功能实验，发现其过表达通过激活MAPK信号通路促进乳腺癌细胞侵袭，提示其作为潜在肿瘤标志物。
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**注**：以上文献为模拟示例，实际研究中需根据具体研究方向查阅权威数据库(如PubMed、ScienceDirect)获取真实文献。
**Background of Recombinant Human Cyclin J (CCNJ) Protein**
Cyclin J (CCNJ), a member of the cyclin protein family, plays a regulatory role in the cell cycle, primarily by interacting with cyclin-dependent kinases (CDKs). Unlike canonical cyclins such as Cyclin D or Cyclin B, CCNJ is classified as a "non-typical" cyclin due to its divergent structural features and distinct expression patterns. It is encoded by the *CCNJ* gene, located on human chromosome 10. and is highly conserved across eukaryotes, suggesting fundamental biological importance.
CCNJ is implicated in cell cycle progression, particularly during the G1/S phase transition and DNA damage response. Studies suggest it modulates CDK activity, influencing cell proliferation, apoptosis, and genome stability. Its expression peaks during the S phase and is tightly regulated by transcriptional and post-translational mechanisms. Notably, CCNJ is overexpressed in certain cancers (e.g., breast, liver) and is linked to poor prognosis, potentially serving as a biomarker or therapeutic target.
Recombinant CCNJ protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, enables functional studies, including kinase assays, protein interaction analyses, and mechanistic exploration of its role in diseases. Research remains ongoing to elucidate its precise molecular mechanisms and therapeutic relevance.
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