Recombinant Human CDH15 protein

中文名： 肌微管钙黏附蛋白(CDH15)重组蛋白
别名： CDH15；CDH14；CDH3；Cadherin-15

货号: PA1000-7204

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CDH15
Uniprot No	P55291
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	61-246aa
氨基酸序列	MASMTGGQQMGRGHHHHHHGNLYFQGGEFLPYPLVQIKSDKQQLGSVIYS IQGPGVDEEPRGVFSIDKFTGKVFLNAMLDREKTDRFRLRAFALDLGGST LEDPTDLEIVVVDQNDNRPAFLQEAFTGRVLEGAVPGTYVTRAEATDADD PETDNAALRFSILQQGSPELFSIDELTGEIRTVQVGLDREVVAVYNLTLQ VADMSGDGLTATASAWAHNSRNLVAAALRVVAVAVVVAAVAN
预测分子量	26 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CDH15重组蛋白的模拟参考文献示例(注：文献为虚构示例，建议通过PubMed或Google Scholar检索真实文献)：

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1. **文献名称**：*Expression and Purification of Recombinant CDH15 for Structural Analysis*

**作者**：Smith A, et al.

**摘要**：本研究报道了人源CDH15胞外域的重组表达及纯化方法，利用哺乳动物表达系统获得高纯度蛋白，并通过X射线晶体学解析其三维结构，揭示了其钙依赖性细胞粘附的关键结构域。

2. **文献名称**：*CDH15 Recombinant Protein Restores Neuromuscular Junction Integrity in a Mouse Model*

**作者**：Chen L, et al.

**摘要**：通过在大鼠模型中注射重组CDH15蛋白，研究证实其能够修复因CDH15缺失导致的神经肌肉连接缺陷，为先天性肌营养不良的蛋白替代疗法提供了实验依据。

3. **文献名称**：*Interaction of Recombinant CDH15 with β-Catenin: Implications for Cell Adhesion Signaling*

**作者**：Yamamoto K, et al.

**摘要**：利用重组CDH15蛋白进行体外结合实验，发现其与β-连环蛋白(β-catenin)的相互作用依赖于特定的细胞内结构域，揭示了CDH15在Wnt信号通路中的潜在调控机制。

4. **文献名称**：*Functional Characterization of CDH15 Mutants Using Recombinant Protein Assays*

**作者**：Gomez R, et al.

**摘要**：通过表达携带致病突变的CDH15重组蛋白，分析其粘附功能及稳定性，发现某些突变导致蛋白错误折叠，为相关遗传性疾病的分子机制提供了新见解。

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**注意**：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“CDH15 recombinant protein”或“CDH15 expression”获取。

背景信息

CDH15 (Cadherin-15), also known as M-cadherin, is a calcium-dependent cell adhesion protein belonging to the cadherin superfamily. It plays a critical role in mediating cell-cell interactions, particularly in developing skeletal muscles and the nervous system. Structurally, CDH15 features extracellular cadherin repeats that facilitate homophilic binding, a transmembrane domain, and a cytoplasmic tail that interacts with catenins (e.g., β-catenin) to link the actin cytoskeleton. This interaction is essential for maintaining tissue integrity, regulating myoblast fusion during muscle formation, and guiding neuronal connectivity.

Recombinant CDH15 protein is engineered using expression systems (e.g., mammalian cells, bacteria) to produce purified, functional forms of the protein for research. Its production often includes tags (e.g., His-tag) for ease of purification or detection. Recombinant CDH15 enables studies on cadherin-mediated adhesion mechanisms, muscle regeneration, and neural circuit assembly. It is also used to investigate diseases linked to CDH15 dysfunction, such as congenital muscular dystrophy and neurodevelopmental disorders. Additionally, it serves as a tool for screening therapeutic agents targeting cadherin-related pathways or cellular interactions. Mutant variants of recombinant CDH15 help dissect structure-function relationships or disease-associated mutations. By providing a controlled source of the protein, recombinant CDH15 advances both basic research and translational applications in cell biology and regenerative medicine.