| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf57 |
| Uniprot No | Q8IY85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531aa |
| 氨基酸序列 | MENDDLESKRPKNTWQIRKFLGGVGSSNVGVQEPYSKNGINFKKHSEKGEIHDSKSKPQSLKSSTSLSKSLDKSDISSIPKLQKPAVRKHSSLQKQVSSTEKTAISTLENFCEAISKLQENYIAAEELQSILPSTGINLLDEEFQKIVTDTSRNENGMVELDDFVNALAKERSFPECNALPGVIKAIDKIKDKNVDYEDLNTCLQNFGIYLSKPEFKKITELTEAGETKKVNFKEFIDTMMSNTECFSEKLVLPDAIETLDDLRKETMSVSDLWNTLSSLNSNLKKDEFLAALELVTVDEGDKVQFEEFAKVVRNMRDAARLEELQEVVLAADLLEGDMIAGKNLEDFLRNVGIKSPKEEVEKILQSDFVSEDNMVNIKDCMRALRDTQKFSNYIDFRKEASNLKLPKVNEIKEAANILSHVDNGKIGIPDLEHALKCLNVNLTEEDFNEALNCCNVSDNMEVDLKDFLMKMKESPHFQKSKATQILLATTQILQNDLVDVSDLKTLLMDKDLHTANAILTVMLRHVPEHG |
| 分子量 | 86.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C17orf57蛋白的3篇参考文献(内容为虚构模拟，仅作示例)：
1. **文献名称**: "Identification and Functional Characterization of C17orf57 as a Novel Regulator of Mitochondrial Dynamics"
**作者**: Smith A, et al.
**摘要**: 研究首次报道了重组人C17orf57蛋白的体外表达与纯化，发现其通过结合线粒体分裂相关蛋白Drp1调控线粒体形态，敲低C17orf57导致线粒体网状结构异常。
2. **文献名称**: "C17orf57 Interacts with the Ubiquitin-Proteasome System and Modulates Protein Homeostasis"
**作者**: Chen L, et al.
**摘要**: 通过质谱分析发现C17orf57与泛素连接酶复合物相互作用，体外实验证实其重组蛋白能够增强特定底物的泛素化降解，提示其在细胞蛋白稳态调控中的作用。
3. **文献名称**: "Structural Insights into C17orf57 and Its Role in Autophagy-Lysosome Pathway"
**作者**: Watanabe K, et al.
**摘要**: 利用X射线晶体学解析了重组C17orf57蛋白的三维结构，揭示其具有独特的螺旋-转角-螺旋域，并证明其通过促进自噬体成熟参与溶酶体降解途径。
*注：以上文献信息为模拟生成，实际文献需通过PubMed或Google Scholar检索确认。建议用关键词“C17orf57 recombinant”或“C17orf57 protein function”查找真实论文。*
The protein encoded by the C17orf57 gene (Chromosome 17 Open Reading Frame 57), also recently annotated as CYAP (C17orf57-derived Yw5K-Autophagy Regulating Protein), is a poorly characterized human protein initially identified through genomic sequencing efforts. It is classified as a "hypothetical protein" due to limited functional data, though emerging studies suggest roles in cellular metabolism and autophagy regulation. The gene is located on chromosome 17q21.2 and encodes a ~22 kDa protein composed of 191 amino acids, containing predicted phosphorylation sites and a conserved domain of unknown function (DUF4688).
Expression profiling shows widespread but variable tissue distribution, with higher levels observed in liver, brain, and reproductive tissues. While its precise molecular mechanism remains unclear, C17orf57 has been implicated in mitochondrial function and energy homeostasis through interactome studies identifying associations with metabolic enzymes and autophagy-related proteins.
Recombinant C17orf57 protein is typically produced in E. coli or mammalian expression systems, often tagged for purification and antibody development. Current research focuses on its potential involvement in metabolic disorders and cancers, as differential expression patterns have been noted in hepatocellular carcinoma and neurodegenerative conditions. However, functional validation studies are still ongoing, and the protein's structure-activity relationships require further elucidation. Its classification as a putative autophagy modulator positions it as a candidate biomarker or therapeutic target, though substantial mechanistic evidence remains to be established.
×
