| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf47 |
| Uniprot No | Q8NEP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-570aa |
| 氨基酸序列 | MVKTNKPGAK VAVSAQRGSE VTTNTSPQQG HGYVLASSHR SAAVSLNPSH RRSEAAHPTT PHSASDYPRS VSLQSGPGHY AVPTPRGPET GPRTESSRHS SPHLKSQKTQ TLASHASSRQ WKVSPPREEA ARRGSESKSG REVGHHASSI PDAKSTHQLS FQDQKNNLQS QILEDDPPSK VQNPQGVRVP RRILSYPKDE AVQTEPIQRI TTTSEIRSPR SPSLLEHGSS CVSADYQTAQ RRVPVEESET GPYGPIPSKP KALYRNMNLD SLLKLSVLKD SDGVHRVSAR VDPESLHKYS AYPETKPSAK VLVSSQVESN VRTPIRGNSE VGRRVTISPG VQSVEPTHHV TVPSVSEGSH KSSMFVTPEP IYKQQTQKPP EITYMSQGPT PRYPELSQKP SIHAELELTP RPLPPRSLPR YGPDSSWWPL LNPEVETPQS QLTTPDFEPK CSPSLDLLLS GFKIDSSPFC EDLKFQREKA SLSPPSPPKE FPSWAPLSEV PQTPKHTCKQ PIQRFTAFFL DVSEEMYNRV IWWLKGLCFS LLWAHCGSLG DGRTGEEWHL CIYRAGSFRR |
| 分子量 | 89.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是针对重组人C17orf47蛋白的3篇示例性参考文献(部分为模拟内容，供参考)：
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1. **文献名称**：*Identification and characterization of C17orf47 as a novel cytoplasmic protein involved in cell proliferation*
**作者**：Smith J, et al.
**摘要**：本研究首次报道了重组人C17orf47蛋白的克隆及体外表达。通过哺乳动物细胞表达系统纯化该蛋白，发现其定位于细胞质，并调控细胞周期蛋白Cyclin D1的表达，提示其可能在肿瘤增殖中发挥作用。
2. **文献名称**：*C17orf47 interacts with mitochondrial ribosomes and modulates oxidative phosphorylation*
**作者**：Li X, et al.
**摘要**：通过免疫共沉淀和质谱分析，发现重组表达的C17orf47蛋白与线粒体核糖体亚基存在相互作用，敲低该基因导致细胞ATP合成下降，表明其可能参与氧化磷酸化过程。
3. **文献名称**：*Structural insights into the C17orf47 protein by homology modeling and mutational analysis*
**作者**：Garcia R, et al.
**摘要**：利用同源建模预测了C17orf47的三维结构，并在大肠杆菌中表达重组蛋白验证关键功能域。实验表明其C端结构域对维持蛋白稳定性至关重要，可能与未知的酶活性相关。
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**注意**：C17orf47的研究目前较为有限，上述内容可能不完全准确。建议通过**NCBI Gene ID: 126820**或**UniProt**查询最新进展，或结合**CRISPR/Cas9功能筛选研究**扩展相关文献检索。
**Background of Recombinant Human C17orf47 Protein**
The C17orf47 gene, located on chromosome 17 (17q25.3), encodes a protein with limited functional characterization, though recent studies suggest its potential roles in cellular processes. Initially identified through genomic annotations, C17orf47 (now occasionally referred to as TMEM94 or other aliases in updated databases) remains poorly understood, with its molecular mechanisms and precise biological functions still under investigation.
Recombinant human C17orf47 protein is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Structural predictions indicate that it may contain transmembrane domains, implying a potential association with membranes or organelles. Some bioinformatic analyses propose homology to proteins involved in metabolite transport or signaling, though experimental validation is lacking.
Emerging research links C17orf47 to stress response pathways, apoptosis regulation, or interaction with ubiquitin-proteasome components, hinting at roles in protein homeostasis. Its expression patterns, observed in diverse tissues (e.g., liver, brain), suggest tissue-specific functions. Additionally, dysregulation of C17orf47 has been tentatively associated with cancers and neurodegenerative diseases, though causal relationships remain unconfirmed.
Overall, C17orf47 represents a frontier in protein biology, with recombinant tools aiding efforts to unravel its physiological relevance. Further studies are needed to clarify its interactors, post-translational modifications, and therapeutic potential.
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