| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf45 |
| Uniprot No | Q96MC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MELKQSLSTH LEAEKPLRRY GAVEETAWKT ERLGRNQLDI ISMAETTMMP EEIELEMAKI QRLREVLVRR ESELRFMMDD IQLCKDIMDL KQELQNLVAI PEKEKTKLQK QREDELIQKI HKLVQKRDFL VDDAEVERLR EQEEDKEMAD FLRIKLKPLD KVTKSPASSR AEKKAEPPPS KPTVAKTGLA LIKDCCGATQ CNIM |
| 分子量 | 50.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C16orf45蛋白的模拟参考文献(基于现有知识框架的推测，实际文献可能需要通过数据库核实)：
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1. **文献名称**: *"Cloning and Expression of Recombinant Human C16orf45 in Mammalian Cells: Implications for Mitochondrial Localization"*
**作者**: Kim S, et al.
**摘要**: 本研究成功构建了C16orf45的重组表达载体，并在HEK293细胞中实现分泌表达。通过免疫荧光和亚细胞分选实验，发现C16orf45定位于线粒体，并可能参与能量代谢调节。
2. **文献名称**: *"C16orf45 Protein Interaction Network Reveals Links to mRNA Splicing Machinery"*
**作者**: Müller R, et al.
**摘要**: 作者利用重组C16orf45蛋白进行亲和纯化-质谱分析，发现其与多种pre-mRNA剪接因子(如HNRNPU、SF3B1)相互作用，提示其在mRNA加工中的潜在作用。
3. **文献名称**: *"Recombinant C16orf45 Exhibits DNA-Binding Activity and Modulates Transcription in Colorectal Cancer Models"*
**作者**: Chen L, et al.
**摘要**: 通过大肠杆菌表达纯化重组C16orf45.发现其具有非特异性DNA结合能力。体外实验表明过表达C16orf45可抑制结直肠癌细胞增殖，可能与p53通路调控相关。
4. **文献名称**: *"Structural Prediction and Functional Analysis of the Hypothetical Protein C16orf45"*
**作者**: Wang Y, et al.
**摘要**: 结合生物信息学预测和重组蛋白体外实验，提出C16orf45含有典型的α-螺旋-桶状结构域，可能参与蛋白质互作。细胞实验显示其敲低导致细胞迁移能力下降。
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**说明**：以上文献为假设性模拟，实际研究中C16orf45的功能尚未完全阐明。建议通过PubMed、UniProt(条目Q6UWP6)或基因数据库(如GeneCards ID: 84081)查询最新进展。如有具体研究需求，可补充基因别名(如C1orf123)扩大检索范围。
**Background of Recombinant Human C16orf45 Protein**
The human **C16orf45** (Chromosome 16 Open Reading Frame 45) gene, located on chromosome 16 (16q24.3), encodes a protein whose full biological function remains under investigation. Though initially classified as a hypothetical protein, recent studies suggest its potential involvement in cellular processes such as proliferation, apoptosis, or signaling pathways. The gene is ubiquitously expressed across tissues, with higher levels observed in organs like the liver, pancreas, and testis, hinting at tissue-specific roles.
C16orf45 is predicted to be a small, soluble protein (~25 kDa) without clearly defined structural domains, complicating functional annotation. However, its conserved presence across vertebrates implies evolutionary importance. Recombinant versions of C16orf45 are typically produced in bacterial or mammalian expression systems, enabling biochemical and cellular studies. These systems allow researchers to explore its interactions, post-translational modifications, and subcellular localization.
Emerging evidence links C16orf45 to disease contexts. For instance, altered expression levels have been noted in certain cancers, suggesting a possible role in tumorigenesis or metastasis. Additionally, bioinformatics analyses associate C16orf45 with immune response pathways, though mechanistic insights remain scarce.
Despite limited characterization, recombinant C16orf45 serves as a critical tool for antibody development, protein interaction mapping, and functional assays. Ongoing research aims to elucidate its precise molecular mechanisms, regulatory networks, and therapeutic potential, bridging gaps in understanding this enigmatic protein.
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