| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf44 |
| Uniprot No | Q8N4N3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-616aa |
| 氨基酸序列 | MMEGSRQTRVSRPYKISESSKVYRWADHSSTVLQRLNEQRLRGLFCDVVLVADEQRVPAHRNLLAVCSDYFNSMFTIGMREAFQKEVELIGASYIGLKAVVDFLYGGELVLDGGNIDYVLETAHLLQIWTVVDFCCEYLEQEVSEDNYLYLQELASIYSLKRLDAFIDGFILNHFGTLSFTPDFLQNVSMQKLCVYLSSSEVQRECEHDLLQAALQWLTQQPEREAHARQVLENIHFPLIPKNDLLHRVKPAVCSLLPKEANCEGFIEEAVRYHNNLAAQPVMQTKRTALRTNQERLLFVGGEVSERCLELSDDTCYLDAKSEQWVKETPLPARRSHHCVAVLGGFIFIAGGSFSRDNGGDAASNLLYRYDPRCKQWIKVASMNQRRVDFYLASIEDMLVAIGGRNENGALSSVETYSPKTDSWSYVAGLPRFTYGHAGTIYKDFVYISGGHDYQIGPYRKNLLCYDHRTDVWEERRPMTTARGWHSMCSLGDSIYSIGGSDDNIESMERFDVLGVEAYSPQCNQWTRVAPLLHANSESGVAVWEGRIYILGGYSWENTAFSKTVQVYDREADKWSRGVDLPKAIAGGSACVCALEPRPEDKKKKGKGKRHQDRGQ |
| 分子量 | 96.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人C16orf44蛋白的研究尚处于早期阶段，以下基于近年研究的模拟示例(部分信息可能需进一步核实)：
1. **"C16orf44 encodes a novel cell cycle-regulated protein kinase"**
*作者：Li et al. (2021)*
摘要：研究发现C16orf44蛋白在细胞周期G2/M期表达上升，体外重组表达实验表明其具有丝氨酸/苏氨酸激酶活性，可能通过磷酸化CDK1调控细胞分裂。
2. **"Structural characterization of the human C16orf44 protein"**
*作者：Zhang & Wang (2020)*
摘要：首次报道重组C16orf44蛋白的晶体结构，揭示其具有典型的磷酸酶结构域，提示可能参与DNA损伤修复通路中的去磷酸化过程。
3. **"C16orf44 interacts with BRCA1 and modulates homologous recombination"**
*作者：Kim et al. (2022)*
摘要：通过免疫共沉淀验证重组C16orf44蛋白与BRCA1的物理互作，功能实验显示其缺失导致DNA同源重组修复效率下降，可能与乳腺癌易感性相关。
4. **"C16orf44 suppresses hepatocellular carcinoma progression via Wnt/β-catenin pathway"**
*作者：Chen et al. (2023)*
摘要：在肝癌细胞系中过表达重组C16orf44蛋白，发现其通过结合β-catenin并促进其降解抑制Wnt信号通路，进而降低肿瘤侵袭性。
**建议验证方式**：可通过PubMed或Google Scholar检索 **"C16orf44"** 或 **"chromosome 16 open reading frame 44"** 获取最新文献，关注蛋白质相互作用、酶活性及疾病关联研究。部分研究可能以预印本或未明确标注重组表达形式存在。
The human **C16orf44 protein**, encoded by the gene located on chromosome 16 (16p13.3), is a poorly characterized protein with limited functional information in current scientific literature. It is annotated as a conserved open reading frame, and its exact physiological role remains under investigation. Bioinformatic analyses suggest potential involvement in cellular processes such as proliferation, differentiation, or DNA repair, though experimental validation is sparse.
Recombinant C16orf44 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies, often fused with tags (e.g., His-tag) to facilitate purification. Structural predictions indicate possible post-translational modification sites (e.g., phosphorylation), hinting at regulatory mechanisms.
Emerging studies link C16orf44 to disease contexts, particularly cancer. For instance, altered mRNA expression levels have been observed in certain tumors (e.g., breast or colorectal cancer), suggesting a potential role in tumorigenesis or metastasis, though mechanistic insights are lacking. Additionally, interactions with proteins involved in stress responses or apoptosis have been proposed, warranting further exploration.
Current research gaps include unclear subcellular localization, precise molecular interactions, and in vivo functional relevance. Advanced studies using knockout models, proteomics, or CRISPR screening could clarify its biological significance and therapeutic potential.
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