关键信息
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基因名
RABIF
- 应用
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别名
RABIF;MSS4;RASGRF3;Guanine nucleotide exchange factor MSS4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P47224
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表达区间
1-123aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE
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分子量
16 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RABIF, a member of the RAS oncogene family, plays a crucial role in intracellular trafficking and membrane dynamics. It is particularly involved in the regulation of vesicle transport, influencing various cellular processes such as endocytosis and exocytosis. Research has indicated that RABIF may interact with several protein partners, suggesting its potential involvement in critical signaling pathways related to cell growth and differentiation. The interest in RABIF has increased due to its implications in diseases, particularly cancer, where dysregulation of vesicular transport can contribute to tumor progression and metastasis. Studies focusing on RABIF recombinantly expressed proteins have aimed to elucidate its functional mechanisms, interactions, and potential as a therapeutic target. By employing techniques such as protein crystallography and biochemical assays, researchers strive to uncover the structural and functional characteristics of RABIF that could lead to novel insights into its role in health and disease. Understanding RABIF's function at a molecular level could provide a foundation for developing new strategies in targeting cancer and other diseases associated with RABIF dysregulation.
